ProtParam

User-provided sequence:

        10         20         30         40         50         60 
SPEPSTKVSE EAESQQWDTS KGDQVSQNGL PAEQGSPRVS YRSQTYQNYK NFNSRRTASD 


HSWSGL 

References and documentation are available.

Number of amino acids: 66

Molecular weight: 7407.80

Theoretical pI: 5.66

Amino acid composition: 
Ala (A)   3	  4.5%
Arg (R)   4	  6.1%
Asn (N)   4	  6.1%
Asp (D)   3	  4.5%
Cys (C)   0	  0.0%
Gln (Q)   7	 10.6%
Glu (E)   5	  7.6%
Gly (G)   4	  6.1%
His (H)   1	  1.5%
Ile (I)   0	  0.0%
Leu (L)   2	  3.0%
Lys (K)   3	  4.5%
Met (M)   0	  0.0%
Phe (F)   1	  1.5%
Pro (P)   4	  6.1%
Ser (S)  13	 19.7%
Thr (T)   4	  6.1%
Trp (W)   2	  3.0%
Tyr (Y)   3	  4.5%
Val (V)   3	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 7

Atomic composition:

Carbon      C	       313
Hydrogen    H	       476
Nitrogen    N	        96
Oxygen      O	       114
Sulfur      S	         0

Formula: C313H476N96O114
Total number of atoms: 999

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15470
Abs 0.1% (=1 g/l)   2.088

Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 66.84
This classifies the protein as unstable.



Aliphatic index: 29.55

Grand average of hydropathicity (GRAVY): -1.483