ProtParam

User-provided sequence:

        10         20         30         40         50         60 
TFEHDIQALG TQVRQLQEDA ARLQAAYAGD KADDIQKREN EVLEAWKSLL DACEGRRVRL 

        70         80         90        100        110        120 
VDTGDKFRFF SMVRDLMLWM EDVIRQIEAQ EKPRDVSSVE LLMNNHQGIK AEIDACNDSF 

       130        140        150        160        170        180 
TACIELGKAL LARKHYASEE IKEKLLQLTE KRKEMIDKWE DRWEWLRLIL EVHQFSRDAS 

       190        200        210        220        230        240 
VAEAWLLGQE PYLSSREIGQ SVDEVEKLIK RHEAFEKSAA TWDERFSALE RLTTLELLEV 

       250        260        270        280        290        300 
RRQQEEEERK RRPPSPEPSA KVSEEAESQQ WDTSKGDQVS QNGLPAEQGS PRMAGTMETS 

       310        320        330        340        350        360 
EMVNGAAEQR TSSKESSPVP SPTSDRKAKS ALPAQSAATL PARTLETPAA QMEGFLNRKH 

       370        380        390        400        410        420 
EWEAHNKKAS SRSWHNVYCV INNQEMGFYK DAKSAASGVP YHSEVPVSLK EAICEVALDY 

       430        440        450        460        470        480 
KKKKHVFKLR LSDGNEYLFQ AKDDDEMNTW IQAITSAISS DKHDTSASTQ STPASSRAQT 

       490        500        510 
LPTSVVTITS ESSPGKREKD KEKDKEKRFS LFGKKK 

References and documentation are available.

Number of amino acids: 516

Molecular weight: 58595.63

Theoretical pI: 5.69

Amino acid composition: 
Ala (A)  50	  9.7%
Arg (R)  34	  6.6%
Asn (N)  13	  2.5%
Asp (D)  31	  6.0%
Cys (C)   5	  1.0%
Gln (Q)  28	  5.4%
Glu (E)  58	 11.2%
Gly (G)  19	  3.7%
His (H)  11	  2.1%
Ile (I)  18	  3.5%
Leu (L)  41	  7.9%
Lys (K)  44	  8.5%
Met (M)  11	  2.1%
Phe (F)  14	  2.7%
Pro (P)  19	  3.7%
Ser (S)  50	  9.7%
Thr (T)  25	  4.8%
Trp (W)  11	  2.1%
Tyr (Y)   8	  1.6%
Val (V)  26	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 89
Total number of positively charged residues (Arg + Lys): 78

Atomic composition:

Carbon      C	      2546
Hydrogen    H	      4058
Nitrogen    N	       736
Oxygen      O	       819
Sulfur      S	        16

Formula: C2546H4058N736O819S16
Total number of atoms: 8175

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    72670
Abs 0.1% (=1 g/l)   1.240, assuming all pairs of Cys residues form cystines


Ext. coefficient    72420
Abs 0.1% (=1 g/l)   1.236, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is T (Thr).

The estimated half-life is: 7.2 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 56.73
This classifies the protein as unstable.



Aliphatic index: 68.90

Grand average of hydropathicity (GRAVY): -0.818