ProtParam
User-provided sequence:
10 20 30 40 50 60
VHSPSNMSMT SSTRLSTKTT DRCLQHFYGP NHEHCFNRTL LRNSIGCEER SDEYRTEFTT
70 80 90 100 110 120
ALQRVDLFMG RLNHVLLTSI STFIKGDLTI ANLGTYEGRF MQVVLFRTRQ FNPHVNFLLG
130
SYPVSAEVIV EH
References and
documentation are available.
Number of amino acids: 132
Molecular weight: 15186.22
Theoretical pI: 7.96
Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 13
Atomic composition:
Carbon C 668
Hydrogen H 1043
Nitrogen N 193
Oxygen O 199
Sulfur S 7
Formula: C
668H
1043N
193O
199S
7
Total number of atoms: 2110
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 6085
Abs 0.1% (=1 g/l) 0.401, assuming all pairs of Cys residues form cystines
Ext. coefficient 5960
Abs 0.1% (=1 g/l) 0.392, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is V (Val).
The estimated half-life is: 100 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.81
This classifies the protein as stable.
Aliphatic index: 78.18
Grand average of hydropathicity (GRAVY): -0.290