ProtParam

User-provided sequence:

        10         20         30         40         50         60 
WLIPITFLTI GYGDVVPGTL WGKIVCLCTG VMGVCCTALL VAVVARKLEF NKAEKHVHNF 

        70 
MMDIHYAKEM KES 

References and documentation are available.

Number of amino acids: 73

Molecular weight: 8141.80

Theoretical pI: 7.87

Amino acid composition: 
Ala (A)   5	  6.8%
Arg (R)   1	  1.4%
Asn (N)   2	  2.7%
Asp (D)   2	  2.7%
Cys (C)   4	  5.5%
Gln (Q)   0	  0.0%
Glu (E)   4	  5.5%
Gly (G)   6	  8.2%
His (H)   3	  4.1%
Ile (I)   5	  6.8%
Leu (L)   7	  9.6%
Lys (K)   6	  8.2%
Met (M)   4	  5.5%
Phe (F)   3	  4.1%
Pro (P)   2	  2.7%
Ser (S)   1	  1.4%
Thr (T)   5	  6.8%
Trp (W)   2	  2.7%
Tyr (Y)   2	  2.7%
Val (V)   9	 12.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 6
Total number of positively charged residues (Arg + Lys): 7

Atomic composition:

Carbon      C	       372
Hydrogen    H	       588
Nitrogen    N	        92
Oxygen      O	        96
Sulfur      S	         8

Formula: C372H588N92O96S8
Total number of atoms: 1156

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14230
Abs 0.1% (=1 g/l)   1.748, assuming all pairs of Cys residues form cystines


Ext. coefficient    13980
Abs 0.1% (=1 g/l)   1.717, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is W (Trp).

The estimated half-life is: 2.8 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            2 min (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.20
This classifies the protein as stable.



Aliphatic index: 106.71

Grand average of hydropathicity (GRAVY): 0.577