ProtParam
User-provided sequence:
10 20 30 40 50 60
YKGPTELTES QKYNFRNDGR CHYMTIHNVT PDDEGVYSVI ARLEPRGEAR STAELYLTTK
70 80 90 100 110 120
EIKLEMKPPD IPDSRVPIPT MPIRAVPPEE IPPAVAPSIP LLLPLPEEKK PPAKRIEVTK
130 140 150 160 170 180
KGVKKDTKKV VAKPKEEAPP PPPVPEVAKK PPPPTPMIPA KPSEIIDVSS KAEEVKITTI
190 200 210 220 230 240
TRKKEIHKEK EAVYERKEAV YEKKVLIEPW EEPYEELETE PYTEPYEEPY YEEPDEDYEE
250 260 270 280 290 300
IKVEAKKEVH EEWEEDYEEG QEYYEREEGY DEGEEEWEEI YHEREIIQVQ KEVHEDLHEK
310 320 330 340 350 360
KIPAKVPEKK VPPPKVPEAP KKPAPEEKTA VPVAKKKEAP PPKVPTAEKK VRKLLPEPKP
370 380 390 400 410 420
QPKEEVVLKS VLRKRPEEEE PKVEPKKVEK VKKPEEPPPP PKAVEVEAPP EPKPKERKVP
430 440 450 460 470 480
EPTKVPEIKP AIPLPGPEPK PKPEPEVKTM KAPPIEPAPT PIAAPVTAPV VGKKAEAKPK
490 500 510 520 530 540
DEAAKPKGPI KGVAKKTPSP IEAERKKLRP GSGGEKPPDE APFTYQLKAV PLKFVKEIKD
IVLTE
References and
documentation are available.
Number of amino acids: 545
Molecular weight: 61684.13
Theoretical pI: 5.65
Total number of negatively charged residues (Asp + Glu): 113
Total number of positively charged residues (Arg + Lys): 102
Atomic composition:
Carbon C 2802
Hydrogen H 4495
Nitrogen N 709
Oxygen O 836
Sulfur S 6
Formula: C
2802H
4495N
709O
836S
6
Total number of atoms: 8848
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 0.726, assuming all pairs of Cys residues form cystines
Ext. coefficient 44810
Abs 0.1% (=1 g/l) 0.726, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is Y (Tyr).
The estimated half-life is: 2.8 hours (mammalian reticulocytes, in vitro).
10 min (yeast, in vivo).
2 min (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 65.35
This classifies the protein as unstable.
Aliphatic index: 67.23
Grand average of hydropathicity (GRAVY): -1.068