ID 1.1.3.23 DE Thiamine oxidase. AN Thiamine dehydrogenase. CA Thiamine + 2 O(2) + H(2)O = thiamine acetic acid + 2 H(2)O(2). CF FAD. CC -!- The product differs from thiamine in replacement of -CH(2)-CH(2)-OH CC by -CH(2)-COOH; the two-step oxidation proceeds without the release CC of the intermediate aldehyde from the enzyme. //