ID   1.10.3.4
DE   o-aminophenol oxidase.
AN   Isophenoxazine synthase.
CA   2 2-aminophenol + O(2) + oxidant = 2-aminophenoxazin-3-one + 2 H(2)O +
CA   reduced oxidant.
CF   Copper or manganese; Flavoprotein.
CC   -!- While the enzyme from the plant Tecoma stans is activated by Mn(2+),
CC       that from the bacterium Streptomyces griseus (GriF) requires Cu(2+)
CC       for maximal activity.
CC   -!- Two molecules of the product 6-iminocyclohexa-2,4-dienone (i.e. 1,2-
CC       benzoquinone monoimine) spontaneously condense with oxidation to
CC       yield 2-aminophenoxazin-3-one.
CC   -!- 3-amino-4-hydroxybenzaldehyde, which has a -CHO group at the para-
CC       position with respect to the hydroxy group of 2-aminophenol,
CC       was found to be the best substrate for GriF.
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