ID   1.14.13.88
DE   Flavonoid 3',5'-hydroxylase.
AN   F3',5'H.
AN   F3'5'H.
CA   (1) A flavanone + NADPH + O(2) = a 3'-hydroxyflavanone + NADP(+) + H(2)O.
CA   (2) A 3'-hydroxyflavanone + NADPH + O(2) = a 3',5'-dihydroxyflavanone +
CA   NADP(+) + H(2)O.
CF   Heme-thiolate.
CC   -!- The 3',5'-dihydroxyflavanone is formed via the 3'-hydroxyflavanone.
CC   -!- In Petunia hybrida the enzyme acts on naringenin, eriodictyol,
CC       dihydroquercetin (taxifolin) and dihydrokaempferol (aromadendrin).
CC   -!- The enzyme catalyzes the hydroxylation of 5,7,4'-trihydroxyflavanone
CC       (naringenin) at either the 3' position to form eriodictyol or at both
CC       the 3' and 5' positions to form 5,7,3',4',5'-pentahydroxyflavanone
CC       (dihydrotricetin).
CC   -!- The enzyme also catalyzes the hydroxylation of
CC       3,5,7,3',4'-pentahydroxyflavanone (taxifolin) at the 5' position,
CC       forming ampelopsin.
CC   -!- NADH is not a good substitute for NADPH.
PR   PROSITE; PDOC00081;
DR   P48418, C75A1_PETHY;  P37120, C75A2_SOLME;  P48419, C75A3_PETHY;
DR   Q96581, C75A4_GENTR;  Q96418, C75A5_EUSGR;  O04773, C75A6_CAMME;
DR   O04790, C75A7_EUSGR;
//