Sample Preparation and Post-separation Analysis

P0AFG3

General information about the entry
View entry in simple text format
Entry name	ODO1_ECOLI
Primary accession number	P0AFG3
Secondary accession number(s)	P07015
integrated into SWISS-2DPAGE on	August 1, 1995 (release 2)
2D Annotations were last modified on	May 15, 2003 (version 1)
General Annotations were last modified on	January 31, 2008 (version 11)
Name and origin of the protein
Description	2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) (Alpha-ketoglutarate dehydrogenase).
Gene name	Name=sucA OrderedLocusNames=b0726, JW0715
Annotated species	Escherichia coli [TaxID: 562]
Taxonomy	Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
References
[1]   MAPPING ON GEL MEDLINE=96314059; PubMed=8740179; [NCBI, ExPASy, EBI, Israel, Japan] Pasquali C., Frutiger S., Wilkins M.R., Hughes G.J., Appel R.D., Bairoch A., Schaller D., Sanchez J.-C., Hochstrasser D.F. ''Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database'' Electrophoresis 17:547-555(1996) [2]   MAPPING ON GEL Vanbogelen R.A., Abshire K.Z., Pertsemlidis A., Clark R.L., Neidhardt F.C. ''Gene-protein database of Escherichia coli K-12, edition 6'' (IN) Neidhardt et al. (eds.)Escherichia coli and Salmonella: Cellular and Molecular Biology (2nd ed.), pp.2067-2117, ASM Press (1996) [3]   PROTEIN EXPRESSION MEDLINE=83160794; PubMed=6339477; [NCBI, ExPASy, EBI, Israel, Japan] Smith M.W., Neidhardt F.C. ''Proteins induced by aerobiosis in Escherichia coli'' J. Bacteriol. 154:344-350(1983)
Comments
SUBUNIT: HOMODIMER
2D PAGE maps for identified proteins
How to interpret a protein
ECOLI {Escherichia coli} Escherichia coli   map experimental info   protein estimated location		ECOLI MAP LOCATIONS: SPOT 2D-000KAU: pI=6.01; Mw=102963 EXPRESSION: INCREASED DURING AEROBIC GROWTH [3]. MAPPING (identification): GEL MATCHING [1] AND IDENTIFIED ON 2-D GELS BY VANBOGELEN [2].
Copyright
This SWISS-2DPAGE entry is copyright the Swiss Institute of Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified and this statement is not removed. Usage by and for commercial entities requires a license agreement (See http://www.expasy.org/ch2d/license.html or send email to license@isb-sib.ch).
Cross-references
ECO2DBASE	G097.0; 6TH EDITION.
UniProtKB/Swiss-Prot	P0AFG3; ODO1_ECOLI.
2D PAGE maps for unidentified proteins
How to interpret a protein map You may obtain an estimated location of the protein on various 2D PAGE maps, provided the whole amino acid sequence is known. The estimation is obtained according to the computed protein's pI and Mw. Warning 1: the displayed region reflects an area around the theoretical pI and molecular weight of the protein and is only provided for the user's information. It should be used with caution, as the experimental and theoretical positions of a protein may differ significantly. Warning 2: the 2D PAGE map is built on demand. This may take some few seconds to be computed.
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Dictyostelium discoideum (Slime mold)	Dictyostelium discoideum
Escherichia coli	Escherichia coli DIGE (4.5-6.5) Escherichia coli(4-5) Escherichia coli(4.5-5.5) Escherichia coli(5-6) Escherichia coli(5.5-6.7) Escherichia coli(6-11) Escherichia coli(6-9)
Homo sapiens (Human)	Colorectal epithelia cells Cerebrospinal Fluid Colorectal adenocarcinoma cell line (DL-1) Erythroleukemia Cell HepG2 Secreted Proteins HepG2 Promyelocytic leukemia cells Kidney Liver Lymphocytes Lymphoma Soluble nuclear proteins and matrix from liver tissue SDS-PAGE of nucleolar proteins from Human HeLa cells 2D-PAGE of nucleolar proteins from Human HeLa cells Plasma Platelet Red blood cells Macrophage Like Cell Line
Mus musculus (Mouse)	Brown adipose tissue Pancreatic islet cells Liver Gastrocnemius muscle Soluble nuclear proteins and matrix from liver tissue White adipose tissue
Saccharomyces cerevisiae (Baker's yeast)	Saccharomyces cerevisiae
Staphylococcus aureus (strain N315)	Staphylococcus aureus