PeptideMass

Enter a UniProtKB protein identifier, ID (e.g. ALBU_HUMAN), or accession number, AC (e.g. P04406), or an amino acid sequence (e.g. 'SELVEGVIV'; you may specify post-translational modifications, but PLEASE read this document first!):

the fields. the cleavage of the protein.

The peptide masses are

with cysteines treated with: nothing (in reduced form) Iodoacetic acid Iodoacetamide 4-vinyl pyridene

with acrylamide adducts

with methionines oxidized

[M+H]⁺ or [M] or [M-H]^- .

average or monoisotopic.

Select an enzyme : Trypsin Trypsin (C-term to K/R, even before P) Trypsin (higher specificity) Trypsin/CNBr Lys C Lys N CNBr Arg C Asp N Asp N + N-terminal Glu Asp N / Lys C Asp N + N-terminal Glu / Lys C Glu C (bicarbonate) Glu C (phosphate) Glu C (phosphate) + Lys C Chymotrypsin (C-term to F/Y/W/M/L, not before P) Chymotrypsin (C-term to F/Y/W, not before P) Trypsin/Chymotrypsin (C-term to K/R/F/Y/W, not before P) Pepsin (pH 1.3) Pepsin (pH > 2) Proteinase K Thermolysin No cutting

Allow for 0 1 2 3 4 5 missed cleavages.
Display the peptides with a mass bigger than 0 500 750 1000 1250 1500 1750 Dalton

sorted by peptide masses or in chronological order in the protein.

For UniProtKB (Swiss-Prot/TrEMBL) entries only:
For each peptide display
all known post-translational modifications,
all database conflicts,
all variants (polymorphisms ),
all mRNA variants (due to alternative splicing, initiation or promoter usage).