FUNCTION: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes (By similarity).
CATALYTIC ACTIVITY: NADPH + acceptor = NADP⁺ + reduced acceptor.
COFACTOR: FMN (By similarity).
SUBUNIT: Homotetramer (By similarity).
SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AM263198; CAK21837.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_850616.1; -.

3D structure databases

ModBase

A0ALF5.

Ontologies

GO:0003959;	Molecular function: NADPH dehydrogenase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01614; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR001155; OxRdtase_FMN_N.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF00724; Oxidored_FMN; 1.
Pfam graphical view of domain structure.

BLOCKS

A0ALF5.

Genome annotation databases

GeneID

4464713; -.

GenomeReviews

AM263198_GR; lwe2419.

KEGG

lwe:lwe2419; -.

NMPDR

fig|386043.6.peg.2350; -.

CMR

A0ALF5; lwe2419.

Other

ProtoNet

A0ALF5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 338`	`338`	`NADPH dehydrogenase.`	`PRO_1000069458`
`NP_BIND`	`22 26`	`5`	`FMN (By similarity).`
`BINDING`	`27 27`		`Substrate (By similarity).`
`BINDING`	`163 163`		`Substrate (By similarity).`
`BINDING`	`166 166`		`Substrate (By similarity).`
`BINDING`	`214 214`		`FMN (By similarity).`
`BINDING`	`307 307`		`FMN (By similarity).`

Sequence information

Length: 338 AA [This is the length of the unprocessed precursor]

Molecular weight: 36966 Da [This is the MW of the unprocessed precursor]

CRC64: 983EB10CB1DE0572 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSKLFSEYKL KDVTLKNRIV MSPMCMYSVE NKDGIATDFH FAHYVSRAAG GTGLVILEAT 

        70         80         90        100        110        120 
AVQEVGRISE FDLGLWNDEQ VPALKHLVDG LHSHGAKAGI QLAHAGRKAV LPGEIVAPSA 

       130        140        150        160        170        180 
IAYDEKSAKP VELTKEAIKE VVADFKRAAY RAKEAGFDVI EIHAAHGYLI HQFLSPITNR 

       190        200        210        220        230        240 
REDNYGGPAG NRYKILSDII KAVKEVWDGP IIVRVSATDY AHGGLQLEDY IPFAKWMKAD 

       250        260        270        280        290        300 
GVELIDVSTG GLVNVAPPVF PGYQVPFADE IRRGAGIATG VLGLITRGEQ AEEILCNERA 

       310        320        330 
DLIIIGRELL RNPYFAKDAA LSLGETIEGP KQYSRAWK

A0ALF5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools