FUNCTION: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).
CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).
SUBUNIT: Interacts with napB (By similarity).
SUBCELLULAR LOCATION: Periplasm (By similarity).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AM286415; CAL11250.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001005483.1; -.

3D structure databases

SMR

A1JL26; 39-828.

ModBase

A1JL26.

Ontologies

GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from HAMAP).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0008940;	Molecular function: nitrate reductase activity (inferred from electronic annotation from HAMAP).
GO:0006777;	Biological process: Mo-molybdopterin cofactor biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01630; -; 1.
PBIL [Tree]

InterPro

IPR009010; Asp_de-COase-like_fold.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR013634; NiFe_hyd_ssu_N.
IPR010051; NO3_reductase_lsu_periplasm.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.

Pfam

PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
PF08425; NiFe_dehyd_N; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01706; NAPA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

A1JL26.

Genome annotation databases

4712672; -.

AM286415_GR; YE1156.

yen:YE1156; -.

fig|630.2.peg.1132; -.

CMR

A1JL26; YE1156.

Other

ProtoNet

A1JL26.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Periplasm; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 31`	`31`	`Tat-type signal (Potential).`
`CHAIN`	`32 831`	`800`	`Periplasmic nitrate reductase.`	`PRO_5000201017`
`METAL`	`47 47`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`50 50`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`54 54`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`82 82`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 831 AA [This is the length of the unprocessed precursor]

Molecular weight: 93407 Da [This is the MW of the unprocessed precursor]

CRC64: C7CFFE972D7248D0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKLSRRDFMK ANAAVAAAAA AGLTIPTVAK AVVGETTNAI KWDKAPCRFC GTGCGVLVGT 

        70         80         90        100        110        120 
QNGRIVASQG DPDSPVNRGL NCVKGYFLPK IMYGKDRLTQ PLLRMKDGQY DKEGDFTPIS 

       130        140        150        160        170        180 
WEKAFDIMEL KFKNALKEKG PTAVGMFGSG QWTVWEGYAA SKLLKAGFRS NNLDPNARHC 

       190        200        210        220        230        240 
MASSVVGFMR TFGMDEPMGC YDDIEEADAF VLWGSNMAEM HPILWSRMTS RRLTNEHVRI 

       250        260        270        280        290        300 
AVLSTFEHRS FELADNPIVF TPQTDLVIMN YIANYIIQNN AVDQGFLDRH VNFRRGATDI 

       310        320        330        340        350        360 
GYGLRPTHPL EKAAKNPGSD ASEPMSFDDF KAFVAEYTLE KTAKMSGVPE DQLESLAQLY 

       370        380        390        400        410        420 
ADPKVKLVSY WTMGFNQHTR GVWANNMCYN LHLLTGKISK PGSGPFSLTG QPSACGTARE 

       430        440        450        460        470        480 
VGTFSHRLPA DMVVTNEKHR QIAETKWQLP AGTIPEKVGL HAVAQDRALK DGTLNAYWVM 

       490        500        510        520        530        540 
CNNNMQAGPN INEERMPGWR DPRNFIVVSD PYPTISALAA DLILPTAMWV EKEGAYGNAE 

       550        560        570        580        590        600 
RRTQFWRQQV PAPGEAKSDL WQMVEFAKRF KVEEVWPDEL INKKPEYRGK TLYDVLFANN 

       610        620        630        640        650        660 
VVNKYPLSEI PADQLNDEAR DFGFYIQKGL FEEYADFGRG HGHDLAPFDR YHQERGLRWP 

       670        680        690        700        710        720 
VVNGKETLWR YREGFDPFVP KGEDVRFYGK PDGKAVIFAL PYEPAAESPD QEYDLWLSTG 

       730        740        750        760        770        780 
RVLEHWHTGS MTRRVPELHR AFPEAVLFIH PLDAKARGLR RGDKVKVISR RGEVISLVET 

       790        800        810        820        830 
RGRNRPPQGL VYMPFFDAAQ LVNNLTLDAT DPLSKETDFK KCAVKLARVV A

A1JL26 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools