NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1371/journal.pgen.0030231; PubMed=18159947 [NCBI, ExPASy, EBI, Israel, Japan]
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.;
"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus.";
PLoS Genet. 3:2515-2528(2007).

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000551; ABM70309.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001009416.1; -.

3D structure databases

ModBase

A2BR98.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

A2BR98.

Genome annotation databases

GeneID

4717736; -.

GenomeReviews

CP000551_GR; A9601_10251.

KEGG

pmb:A9601_10251; -.

CMR

A2BR98; A9601_10251.

Other

ProtoNet

A2BR98.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 390`	`390`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_1000070549`

Sequence information

Length: 390 AA [This is the length of the unprocessed precursor]

Molecular weight: 45687 Da [This is the MW of the unprocessed precursor]

CRC64: 95B341EDB84E11BC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAQSTVESKN RKDINNGKIP AKETILSPRF YTTDFEAMEN MDLSINEEEL EAICEEFRKD 

        70         80         90        100        110        120 
YNRHHFVRNS EFEGAAEKLD PETRELFVDF LEGSCTSEFS GFLLYKELSK RIKVKNPLLA 

       130        140        150        160        170        180 
ECFAHMARDE ARHAGFLNKS MSDFGLQLDL GFLTANKDYT YFPPRSIFYA TYLSEKIGYW 

       190        200        210        220        230        240 
RYIAIYRHLE KNPDSKIFPL FNYFENWCQD ENRHGDFFDA LMKAQPRTVK SLSKKITIGG 

       250        260        270        280        290        300 
STFTHPLFDY FHRFRYFLNN LPLTSKLWSR FFLLAVFATM YARDLGIKKD FYSSLGLDAR 

       310        320        330        340        350        360 
DYDQFVINKT NETAARVFPV VMDVYDNSFY GRLDKIVENN KVLSDIANSD GNKVSKTFKK 

       370        380        390 
LPKYLSNGYQ LLRLYLLKPL DSKDFQPSIR

A2BR98 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools