[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Brain cortex; NIH - Mammalian Gene Collection (MGC) project; Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
CATALYTIC ACTIVITY: (S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate.
CATALYTIC ACTIVITY: 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC149097; AAI49098.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001095357.1; -.

UniGene

Bt.47646

3D structure databases

ModBase

A6QP15.

Ontologies

GO:0005739;	Cellular component: mitochondrion (inferred from sequence or structural similarity from UniProtKB).
GO:0047988;	Molecular function: hydroxyacid-oxoacid transhydrogenase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0015993;	Biological process: molecular hydrogen transport (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR001670; Fe_AlcDHase.
Graphical view of domain structure.

Pfam

PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.

PROSITE

PS00913; ADH_IRON_1; FALSE_NEG.
PS00060; ADH_IRON_2; FALSE_NEG.

BLOCKS

A6QP15.

Genome annotation databases

Ensembl

ENSBTAG00000004476; Bos taurus. [Contig view]

GeneID

507711; -.

KEGG

bta:507711; -.

Other

ProtoNet

A6QP15.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (Potential).`
`CHAIN`	`? 466`		`Hydroxyacid-oxoacid transhydrogenase, mitochondrial.`	`PRO_0000322995`

Sequence information

Length: 466 AA [This is the length of the unprocessed precursor]

Molecular weight: 50343 Da [This is the MW of the unprocessed precursor]

CRC64: EB601252CBE6DA6E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAARSRVVH LLRLLQRAAC QCPSHSHTYS QAPGLSPSGK TTDYAFEMAV STIRYGAGVT 

        70         80         90        100        110        120 
KEVGMDLQSM GAKNVCLMTD KNLSQLPPVQ TVMDSLVKNG INFKVYDHVR VEPTDTSFME 

       130        140        150        160        170        180 
AIEFAKKGAF DAFLAVGGGS TIDTCKAANL YSSSPDSDFL DYVNAPIGKG KPVTVPLKPL 

       190        200        210        220        230        240 
IAVPTTSGTG SETTGVAIFD YEHLKVKTGI ASRAIKPTLG LIDPLHTLHM PERVVANSGF 

       250        260        270        280        290        300 
DVLCHALESY TALPYHMRSP CPSSPITRPA YQGSNPISDI WAVHALRIVA KYLKRAIRNP 

       310        320        330        340        350        360 
DDLEARSNMH LASAFAGIGF GNAGVHLCHG MSYPISGLVK TYKAKDYNVD HPLVPHGLSV 

       370        380        390        400        410        420 
VLTSPAVFTF TSQMFPERHL EVAEILGADT RTARRPDAGP VLADTLRKFL FDLDVDDGLA 

       430        440        450        460 
AIGYSKADIP ELVKGTLPQE RVTKLAPRPQ SEEDLSALFE ASMKLY

A6QP15 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools