NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Ivanova N., Vieille C., Richardson P.;
"Complete sequence of Actinobacillus succinogenes 130Z.";
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate (By similarity).
CATALYTIC ACTIVITY: H₂S + 3 NADP⁺ + 3 H₂O = sulfite + 3 NADPH.
COFACTOR: Binds 1 siroheme per subunit (By similarity).
COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).
SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000746; ABR75038.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001344973.1; -.

3D structure databases

ModBase

A6VPZ1.

Ontologies

GO:0004783;	Molecular function: sulfite reductase (NADPH) activity (inferred from electronic annotation from HAMAP).
GO:0000103;	Biological process: sulfate assimilation (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01540; -; 1.
PBIL [Tree]

InterPro

IPR011786; CysI.
IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
Graphical view of domain structure.

Pfam

PF01077; NIR_SIR; 1.
PF03460; NIR_SIR_ferr; 2.
Pfam graphical view of domain structure.

PR00397; SIROHAEM.

TIGR02041; CysI; 1.

PS00365; NIR_SIR; 1.

Genome annotation databases

GeneID

5349238; -.

GenomeReviews

CP000746_GR; Asuc_1686.

KEGG

asu:Asuc_1686; -.

CMR

A6VPZ1; Asuc_1686.

Other

ProtoNet

A6VPZ1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 588`	`588`	`Sulfite reductase [NADPH] hemoprotein beta-component.`	`PRO_0000318561`
`METAL`	`443 443`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`449 449`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`488 488`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`492 492`		`Iron (siroheme axial ligand) (By similarity).`
`METAL`	`492 492`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 588 AA [This is the length of the unprocessed precursor]

Molecular weight: 66565 Da [This is the MW of the unprocessed precursor]

CRC64: CA199CDA15D40BF1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTDSTKQKGL EWQKQGLSDN ERLKAESNFL RGTILDDLED GLTGGFKGDN FQMIRFHGMY 

        70         80         90        100        110        120 
EQDDRDIRAE RADEKLEARK FMLLRCRLPG GIIKPAQWIE IDKFARDNNY YQSIRLTNRQ 

       130        140        150        160        170        180 
TFQYHGVPKT KLQEMHRLLH KLGLDSIATA SDMNRNVLCS SNPVESELHQ EAYEWAKKIS 

       190        200        210        220        230        240 
EHLLPRTNGY LDVWIAGKKV QSSDSFLGQE DEPILGKTYL PRKYKTAVVL PPLNDVDMYG 

       250        260        270        280        290        300 
NDMNFVGIQD EAGKLVGFNV LVGGGLSFEH GNTKTYPNVA LELGYIPVEQ TLKAAECIVT 

       310        320        330        340        350        360 
TQRDFGNRAD RKNARLRYTL QNMTLDGFRE EVERRMGFKF ESIRPFEFTE RGDRIGWVKG 

       370        380        390        400        410        420 
IDDKWHLTCF IESGRITDKP GKPLMTGMLE LAKVHTGDFR ITANQNIIIA NVAEEDKQRI 

       430        440        450        460        470        480 
EDIAREYGLI GNISKLRENS MSCVSFPTCP LAMAESERAL PEFIDELDNI MAKHDVADDY 

       490        500        510        520        530        540 
IVTRITGCPN GCGRAMLAEI GLVGKAIGRY NLHLGGDRPG TRIPRMYKEN ITLPEILAEL 

       550        560        570        580 
DGLIGRWAKE RNSNEGFGDF VIRAGIIKPV VNAVVDFWDA NLIPTVTA

A6VPZ1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools