NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1371/journal.pgen.0030142; PubMed=17784789 [NCBI, ExPASy, EBI, Israel, Japan]
Eppinger M., Rosovitz M.J., Fricke W.F., Rasko D.A., Kokorina G., Fayolle C., Lindler L.E., Carniel E., Ravel J.;
"The complete genome sequence of Yersinia pseudotuberculosis IP31758, the causative agent of Far East scarlet-like fever.";
PLoS Genet. 3:1508-1523(2007).

Comments

FUNCTION: This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate (By similarity).
CATALYTIC ACTIVITY: H₂S + 3 NADP⁺ + 3 H₂O = sulfite + 3 NADPH.
COFACTOR: Binds 1 siroheme per subunit (By similarity).
COFACTOR: Binds 1 4Fe-4S cluster per subunit (By similarity).
SUBUNIT: Alpha(8)-beta(4). The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).
SIMILARITY: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000720; ABS46664.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001402267.1; -.

3D structure databases

ModBase

A7FLY9.

Ontologies

GO:0004783;	Molecular function: sulfite reductase (NADPH) activity (inferred from electronic annotation from HAMAP).
GO:0000103;	Biological process: sulfate assimilation (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01540; -; 1.
PBIL [Tree]

InterPro

IPR011786; CysI.
IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
Graphical view of domain structure.

Pfam

PF01077; NIR_SIR; 1.
PF03460; NIR_SIR_ferr; 2.
Pfam graphical view of domain structure.

PR00397; SIROHAEM.

TIGR02041; CysI; 1.

PS00365; NIR_SIR; 1.

Genome annotation databases

GeneID

5388312; -.

GenomeReviews

CP000720_GR; YpsIP31758_3310.

KEGG

ypi:YpsIP31758_3310; -.

NMPDR

fig|349747.3.peg.3403; -.

CMR

A7FLY9; YpsIP31758_3310.

Other

ProtoNet

A7FLY9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Heme; Iron; Iron-sulfur; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 576`	`576`	`Sulfite reductase [NADPH] hemoprotein beta-component.`	`PRO_1000068781`
`METAL`	`435 435`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`441 441`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`480 480`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`484 484`		`Iron (siroheme axial ligand) (By similarity).`
`METAL`	`484 484`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 576 AA [This is the length of the unprocessed precursor]

Molecular weight: 64078 Da [This is the MW of the unprocessed precursor]

CRC64: CB5462BD2EA69D23 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNEKHPGPLV VSGKLSDGER MKSESNFLRG TIAEDLNNGL TGGFSGDNFL LIRFHGMYQQ 

        70         80         90        100        110        120 
DDRDIRAERA EQKLEPRHAM MLRCRLPGGI ITPQQWLGID KFAADNTLYG SIRITNRQTF 

       130        140        150        160        170        180 
QFHGILKGNV KPAHQLLNEL GLDALATAND VNRNVLCTSN PVESALHQEA YEWAKKISEH 

       190        200        210        220        230        240 
LLPRTRAYAE IWLDAEKVAT TDEEPILGAT YLPRKFKTTV VIPPQNDVDL HANDLNFVAV 

       250        260        270        280        290        300 
ADKGKLIGFN VLVGGGLSIA HGDKNTYPRK ASEFGYIPLK HTLAIAEAVV TTQRDWGNRT 

       310        320        330        340        350        360 
DRKNAKTKYT LERVGVETFK AEVEKRAGVS FSAIKPYQFT GRGDRIGWVK GVDKKWHLTL 

       370        380        390        400        410        420 
FIENGRLLDY PGRSLKTGVA EIAKIHQGDF RLTANQNLIV AGVPEKDKAR IEALAREHGL 

       430        440        450        460        470        480 
MDDNVTSQRE NSMACVSFPT CPLAMAEAER FLPEFVTRVE GILQQHGLAD EHIVLRVTGC 

       490        500        510        520        530        540 
PNGCGRALLA EVGLVGKAVG RYNLHLGGNR EGTRIPRMYR ENITADEILL ITDQLVGRWA 

       550        560        570 
KERHVDEGFG DFVIRAGVIA PVIDSARDFY DVQEAM

A7FLY9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools