FUNCTION: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes (By similarity).
CATALYTIC ACTIVITY: NADPH + acceptor = NADP⁺ + reduced acceptor.
COFACTOR: FMN (By similarity).
SUBUNIT: Homotetramer (By similarity).
SIMILARITY: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily.

Copyright

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Cross-references

Sequence databases

EMBL

CP000764; ABS21842.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001374837.1; -.

3D structure databases

ModBase

A7GNY4.

Ontologies

GO:0003959;	Molecular function: NADPH dehydrogenase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01614; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR001155; OxRdtase_FMN_N.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF00724; Oxidored_FMN; 1.
Pfam graphical view of domain structure.

BLOCKS

A7GNY4.

Genome annotation databases

GeneID

5345792; -.

GenomeReviews

CP000764_GR; Bcer98_1529.

KEGG

bcy:Bcer98_1529; -.

CMR

A7GNY4; Bcer98_1529.

Other

ProtoNet

A7GNY4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Flavoprotein; FMN; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 346`	`346`	`NADPH dehydrogenase.`	`PRO_0000323520`
`NP_BIND`	`23 27`	`5`	`FMN (By similarity).`
`BINDING`	`28 28`		`Substrate (By similarity).`
`BINDING`	`164 164`		`Substrate (By similarity).`
`BINDING`	`167 167`		`Substrate (By similarity).`
`BINDING`	`215 215`		`FMN (By similarity).`
`BINDING`	`308 308`		`FMN (By similarity).`

Sequence information

Length: 346 AA [This is the length of the unprocessed precursor]

Molecular weight: 38479 Da [This is the MW of the unprocessed precursor]

CRC64: 65ED5C561EBC61F0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNTQLFSPFT IQNVTLKNRI VMSPMCMYSS ENEDGKVTNF HLIHYGTRAM GQVGLVMLEA 

        70         80         90        100        110        120 
TAVAAEGRIS NKDLGIWNDE HIEGLQKTVS FIKEHDSKAA IQLAHAGRKA ELHTDPVAPS 

       130        140        150        160        170        180 
AIPFNDKMKV PVAMSKEQIQ DTITAFQKAA IRSKQAGFDV IELHGAHGYL INEFLSPLSN 

       190        200        210        220        230        240 
KRTDEYGGTA ENRYRFLREI IHAVKEVWEG PLFVRISADD YHPEGLTVND YVQFAKWMKE 

       250        260        270        280        290        300 
QGVDLIDCSS GAVVPAHIDV YPGYQVKYAK HLKEHAQIAT GAVGLITSGV QAEQILVNSE 

       310        320        330        340 
ADLIFVGREL LRNPYFPRTA ANELGFELKD PHQYSRAPGK IANTSK

A7GNY4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools