NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., Nelson K.E.;
"Complete genome sequence of Campylobacter hominis ATCC BAA-381, a commensal isolated from the human gastrointestinal tract.";
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).
CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).
SUBUNIT: Interacts with napB (By similarity).
SUBCELLULAR LOCATION: Periplasm (By similarity).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000776; ABS51551.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001407228.1; -.

3D structure databases

ModBase

A7I3Y7.

Ontologies

GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from HAMAP).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0008940;	Molecular function: nitrate reductase activity (inferred from electronic annotation from HAMAP).
GO:0006777;	Biological process: Mo-molybdopterin cofactor biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from HAMAP).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01630; -; 1.
PBIL [Tree]

InterPro

IPR009010; Asp_de-COase-like_fold.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR010051; NO3_reductase_lsu_periplasm.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.

Pfam

PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01706; NAPA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

A7I3Y7.

Genome annotation databases

GeneID

5408979; -.

GenomeReviews

CP000776_GR; CHAB381_1716.

KEGG

cha:CHAB381_1716; -.

NMPDR

fig|360107.5.peg.1666; -.

CMR

A7I3Y7; CHAB381_1716.

Other

ProtoNet

A7I3Y7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Periplasm; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 29`	`29`	`Tat-type signal (Potential).`
`CHAIN`	`30 920`	`891`	`Periplasmic nitrate reductase.`	`PRO_1000069713`
`METAL`	`42 42`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`45 45`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`49 49`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`77 77`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 920 AA [This is the length of the unprocessed precursor]

Molecular weight: 103807 Da [This is the MW of the unprocessed precursor]

CRC64: 5168FBEB47C95866 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNRRDFIKST AAAAACASAG IALPANLNAE ENHGWRWDKA VCRFCGTGCG IMVATKNGKI 

        70         80         90        100        110        120 
VAVKGDPEAP VNRGLNCIKG YFNAKIMYGD DRITEPLLRV NSKGEFDKHG KFAPVSWKKA 

       130        140        150        160        170        180 
FDVMEKEFKK AYKEKGPTGI AVFGSGQYTI QEGYAAAKLV KAGFRSNNID PNARHCMASA 

       190        200        210        220        230        240 
VVGFMQVFGI DEPSGCFDDI ELTDTIVTWG ANMAEMHPIL WSRVNDRKLS APEKVKVVNL 

       250        260        270        280        290        300 
STFSSRTSSI ADIEIIFRPS TDVAIWNYIA REIVYNHPEA IDTEFLKNCE FATGPVDIGY 

       310        320        330        340        350        360 
GMRNNPNHPK FSEAEKDTVS HQVSKKLSQA EGVSLAYLGL KAGDTLEMKN AKKAGKHWAI 

       370        380        390        400        410        420 
SFEEFKKALA PYTLDYVAEI AKGDENESIE QFKEKLQNLA NLYIEKNRKI VSFWTMGFNQ 

       430        440        450        460        470        480 
HTRGTWVNEQ SYMVHFLLGK QASPGNGAFS LTGQPSACGT AREVGTFCHR LPADMVVANP 

       490        500        510        520        530        540 
KHREITEKIW KLPAGTINPK NGSHFVGLMR DLEDGKVKFA WVQVNNPWHN TANANHWIKA 

       550        560        570        580        590        600 
AREMDNFIVV SDCYPGISAK VADLILPSAM IYEKWGAYGN AERRTQHWRQ QVLPVGDAMS 

       610        620        630        640        650        660 
DTWQILEFAK RFKLKDVWGE QKIDDKLTLP NVLEEAKTMG YDENATLFDI LFANDYYKGF 

       670        680        690        700        710        720 
KPEKIKFDNS EVNGDTRNVK GSDGEVFKGY GFFIQKALWE EYRKFGLGHG HDLADFDTYH 

       730        740        750        760        770        780 
KVRGLKWPVV DGKETSWRFN KKYDPYAKKE ETSGDFAFYG NKNKKLDKGD LIGIKGEDKV 

       790        800        810        820        830        840 
DINNKAKIFF RPYMDPPEIP SEEYPFWLCT GRVLEHWHSG TMTMRVPELY RAVPEALCYM 

       850        860        870        880        890        900 
NPLDAEKLKL SQGDTIWIES RRGKVKVKID TRGRNIPPVG LVYVPWFDEN VFINKVTLDA 

       910        920 
TCPLSSETDY KKCAVKIYKA

A7I3Y7 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools