[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Ascending colon; NIH - Mammalian Gene Collection (MGC) project; Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Irreversible conversion of delta-1-pyrroline-5-carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes (By similarity).
CATALYTIC ACTIVITY: (S)-1-pyrroline-5-carboxylate + NAD(P)⁺ + 2 H₂O = L-glutamate + NAD(P)H.
PATHWAY: Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
SIMILARITY: Belongs to the aldehyde dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC134524; AAI34525.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001099116.1; -.

UniGene

Bt.3248

3D structure databases

ModBase

A7YWE4.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
IPR005931; d-1-pyrroline-5-COlate_DHase-1.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11699; Aldehyde_dehyd; 1.

Pfam

PF00171; Aldedh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01236; D1pyr5carbox1; 1.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; 1.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.

BLOCKS

A7YWE4.

Genome annotation databases

Ensembl

ENSBTAG00000030335; Bos taurus. [Contig view]

GeneID

100126042; -.

KEGG

bta:100126042; -.

Other

ProtoNet

A7YWE4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Acetylation; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Proline metabolism; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 24`	`24`	`Mitochondrion (By similarity).`
`CHAIN`	`25 563`	`539`	`Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial.`	`PRO_0000342182`
`NP_BIND`	`296 301`	`6`	`NAD (By similarity).`
`ACT_SITE`	`314 314`		`Proton acceptor (By similarity).`
`ACT_SITE`	`348 348`		`Nucleophile (By similarity).`
`SITE`	`211 211`	`1`	`Transition state stabilizer (By similarity).`
`MOD_RES`	`99 99`		`N6-acetyllysine (By similarity).`
`MOD_RES`	`114 114`		`N6-acetyllysine (By similarity).`
`MOD_RES`	`402 402`		`N6-acetyllysine (By similarity).`
`MOD_RES`	`505 505`		`Phosphotyrosine (By similarity).`

Sequence information

Length: 563 AA [This is the length of the unprocessed precursor]

Molecular weight: 61495 Da [This is the MW of the unprocessed precursor]

CRC64: E45B568B4844FEF0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLLRSAALCR ALLARRGRAA GLCRRCVSSL QVANEPVLAF TQGSPERDAL QKALKELKGR 

        70         80         90        100        110        120 
TEAIPCVVGD EEVWTSDVRY QASPFNHGHK VAKFCYADKA LLHRAIGAAL AARKEWDLKP 

       130        140        150        160        170        180 
VADRAQVFLK AADLLSGPRR AEVLAKTMVG QGKTVIQAEI DAAAELIDFF RFNAKFAMEL 

       190        200        210        220        230        240 
EGEQPLSVPP STNSMLYRGL EGFVAAISPF NFTAIGGNLA GAPALMGNVV LWKPSDTAML 

       250        260        270        280        290        300 
ASYAVYRILR EAGLPPNIIQ FVPADGPTFG DTVTSSEHLC GINFTGSVPT FKHLWKQVAQ 

       310        320        330        340        350        360 
NLDRFRTFPR LAGECGGKNF HFVHRSADVD SVVSGTLRSA FEYGGQKCSA CSRLYAPRSL 

       370        380        390        400        410        420 
WPQIKGRLLE ELGGIKVGNP AEDFGTFFSA VIDAKSFGRI RKWLEHARSS PSLTILAGGH 

       430        440        450        460        470        480 
CDDSVGYFVE PCIVETKDPQ DPIMKEEIFG PVLAVYVYPD EEYKETLRLV DSTTSYGLTG 

       490        500        510        520        530        540 
AVFAQDKDVL REATELLRHA AGNFYINDKS TGSVVGQQPF GGARASGTND KPGGPHYVLR 

       550        560 
WTSPQVIKET HGPLGDWRYP YMQ

A7YWE4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools