NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nbt1325; PubMed=17704766 [NCBI, ExPASy, EBI, Israel, Japan]
Chen X.H., Koumoutsi A., Scholz R., Eisenreich A., Schneider K., Heinemeyer I., Morgenstern B., Voss B., Hess W.R., Reva O., Junge H., Voigt B., Jungblut P.R., Vater J., Suessmuth R., Liesegang H., Strittmatter A., Gottschalk G., Borriss R.;
"Comparative analysis of the complete genome sequence of the plant growth-promoting bacterium Bacillus amyloliquefaciens FZB42.";
Nat. Biotechnol. 25:1007-1014(2007).

Comments

CATALYTIC ACTIVITY: (S)-dihydroorotate + O₂ = orotate + H₂O₂.
COFACTOR: Binds 1 FMN per subunit (By similarity).
PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6 .
SUBUNIT: Heterotetramer of 2 pyrK and 2 pyrD subunits (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000560; ABS73900.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001421131.1; -.

3D structure databases

ModBase

A7Z4H4.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0004158;	Molecular function: dihydroorotate oxidase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00224; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR012135; DHO_DHase_1_2.
IPR005720; DHO_DHase_1_core.
IPR001295; Dihydroorotate_DHase_core.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF01180; DHO_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000164; DHO_oxidase; 1.

TIGRFAMs

TIGR01037; pyrD_sub1_fam; 1.

PROSITE

PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.

BLOCKS

A7Z4H4.

Genome annotation databases

GeneID

5461856; -.

GenomeReviews

CP000560_GR; RBAM_015370.

KEGG

bay:RBAM_015370; -.

CMR

A7Z4H4; RBAM_015370.

Other

ProtoNet

A7Z4H4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; Flavoprotein; FMN; Oxidoreductase; Pyrimidine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 311`	`311`	`Dihydroorotate dehydrogenase.`	`PRO_1000024121`
`ACT_SITE`	`128 128`		`Nucleophile (By similarity).`

Sequence information

Length: 311 AA [This is the length of the unprocessed precursor]

Molecular weight: 33022 Da [This is the MW of the unprocessed precursor]

CRC64: DFCCFF8D08521A00 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLEVNLPGLQ LKNPIIPASG CFGFGKEYAG FYDLSCLGGI MIKATTNEAR FGNPTPRVAE 

        70         80         90        100        110        120 
TSAGMLNAIG LQNPGLEHVL NHELPWLEQF DTPIIANVAG SQVDDYKEVA EHISQAPNVH 

       130        140        150        160        170        180 
ALELNISCPN VKTGGIAFGT DPVMAAELTK AVKEVSAVPV YVKLSPNVAN ITEIAKAIEA 

       190        200        210        220        230        240 
AGADGLTMIN TLIGMRLDLK TGRPILANKT GGLSGPAIKP VAIRMVYEVS QAVHIPIIGM 

       250        260        270        280        290        300 
GGVQTAEDAL EFLLAGASAV AVGTANFVNP FVCPEIIEQL PRVLRQYGFQ SVSECIGRSW 

       310 
KHEKQPAHHR A

A7Z4H4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools