[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E.; "Genome sequence of Campylobacter concisus 13826 isolated from human feces."; Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).
CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).
SUBUNIT: Interacts with napB (By similarity).
SUBCELLULAR LOCATION: Periplasm (By similarity).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000792; EAT97977.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001466512.1; -.

3D structure databases

ModBase

A7ZCK4.

Ontologies

GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from HAMAP).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0008940;	Molecular function: nitrate reductase activity (inferred from electronic annotation from HAMAP).
GO:0006777;	Biological process: Mo-molybdopterin cofactor biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01630; -; 1.
PBIL [Tree]

InterPro

IPR009010; Asp_de-COase-like_fold.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR010051; NO3_reductase_lsu_periplasm.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.

Pfam

PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01706; NAPA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

A7ZCK4.

Genome annotation databases

GeneID

5597288; -.

GenomeReviews

CP000792_GR; Ccon26_06280.

KEGG

cco:CCC13826_0868; -.

CMR

A7ZCK4; Ccon26_06280.

Other

ProtoNet

A7ZCK4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Periplasm; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 30`	`30`	`Tat-type signal (Potential).`
`CHAIN`	`31 926`	`896`	`Periplasmic nitrate reductase.`	`PRO_1000073640`
`METAL`	`44 44`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`47 47`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`51 51`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`79 79`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 926 AA [This is the length of the unprocessed precursor]

Molecular weight: 104218 Da [This is the MW of the unprocessed precursor]

CRC64: 04D4862157A3E725 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNRRDFIKSA AASAACASAG IAVPSSLSAA NEAEKGWRWD KAACRFCGTG CGIMVATKDG 

        70         80         90        100        110        120 
KIVAVKGDPE APVNRGLNCI KGYFNAKIMY GEDRITHPLL RVNEKGEFDK KGKFKQVSWK 

       130        140        150        160        170        180 
QAFDVMEAQF RKTYDELGPH GVGVLGSGQY TIPEGYAAVK LMKGGFRSNS IDPNARHCMA 

       190        200        210        220        230        240 
SAVLGFMQVF GIDEPSGCFD DIELTDTVIA WGANMAEMHP ILWARVSDRK LSDPDRVKVV 

       250        260        270        280        290        300 
NLSTYSTRTS NLADIEIIFA PSSDLAIWNY IAREIVYNHP EMIDEEFVKK HCVFTAGPVD 

       310        320        330        340        350        360 
IGYGLRPDIH HKKYAPSELD TAATEKSKVL SEAEGVTLSY LGLKAGDTLE NKNAAKADAH 

       370        380        390        400        410        420 
WQITFEEFKK ALAPYTLDFT AKVAKGDPNE DINEFKKKLK ALADLYIEKN RKVVSFWTMG 

       430        440        450        460        470        480 
FNQHQRGTWV NEQAYMVHFL LGKQALPGSG AFSLTGQPSA CGTAREVGTF VHRLPADMVV 

       490        500        510        520        530        540 
GNPKHREITE KLWKLPAGTL SGTPGSHYVK MMRDLEDGKV KFIWVQVNNP WQNTANANHW 

       550        560        570        580        590        600 
IKAAREMDNF IVVSDPYPGI SAKVADLILP TAMIYEKWGA YGNAERRTQH WRQQVLPVGE 

       610        620        630        640        650        660 
AMPDIWQMLE FSKRFKLKDV WGEKKVNDKV TLPSVLEAAK AMGYSEEDTL FDVLFANEDA 

       670        680        690        700        710        720 
KKFSANDPIM ENYDNTEVFG DSRKVIGSDG KEFKGYGFFI HKYLWEEYRK FGVGHGHDLA 

       730        740        750        760        770        780 
DFDTYHRVRG LRWPVVDGKE TQWRFNTKFD PYAKKAAPND KFAFYGNKNA ALPTGDLKGV 

       790        800        810        820        830        840 
KNQEKTPLAN KAKIFFRPYM DPCEMPSKDY PFWLCTGRVL EHWHTGTMTM RVPELYRAVP 

       850        860        870        880        890        900 
EALCYMHEDD AKKLGVLQNE IVWVESRRGK VKARVDLKGR NKPPVGLVYV PFFDENVFIN 

       910        920 
KVCLDATCPI SKETDYKKCA VKIYKA

A7ZCK4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools