[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Uterus; NIH - Mammalian Gene Collection (MGC) project; Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively (By similarity).
CATALYTIC ACTIVITY: N⁶-(L-1,3-dicarboxypropyl)-L-lysine + NADP⁺ + H₂O = L-lysine + 2-oxoglutarate + NADPH.
CATALYTIC ACTIVITY: N⁶-(L-1,3-dicarboxypropyl)-L-lysine + NAD⁺ + H₂O = L-glutamate + 2-aminoadipate 6-semialdehyde + NADH.
PATHWAY: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 1/6 .
PATHWAY: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: In the N-terminal section; belongs to the AlaDH/PNT family.
SIMILARITY: In the C-terminal section; belongs to the saccharopine dehydrogenase family.

Copyright

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Cross-references

Sequence databases

EMBL

BC153851; AAI53852.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001103267.1; -.

UniGene

Bt.39765

3D structure databases

ModBase

A8E657.

Family and domain databases

InterPro

IPR007698; Ala_DHase/PNT_C.
IPR008142; Ala_DHase/PNT_CS1.
IPR008143; Ala_DHase/PNT_CS2.
IPR007886; Ala_DHase/PNT_N.
IPR005097; Saccharopine_DHase.
Graphical view of domain structure.

Pfam

PF01262; AlaDh_PNT_C; 1.
PF05222; AlaDh_PNT_N; 1.
PF03435; Saccharop_dh; 1.
Pfam graphical view of domain structure.

PROSITE

PS00836; ALADH_PNT_1; FALSE_NEG.
PS00837; ALADH_PNT_2; FALSE_NEG.

BLOCKS

A8E657.

Genome annotation databases

Ensembl

ENSBTAG00000012128; Bos taurus. [Contig view]

GeneID

520865; -.

KEGG

bta:520865; -.

Other

ProtoNet

A8E657.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; Multifunctional enzyme; NAD; NADP; Oxidoreductase; Transit peptide.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 32`	`32`	`Mitochondrion (By similarity).`
`CHAIN`	`33 926`	`894`	`Alpha-aminoadipic semialdehyde synthase, mitochondrial.`	`PRO_0000315867`
`REGION`	`33 455`	`423`	`Lysine-ketoglutarate reductase (By similarity).`
`REGION`	`477 926`	`450`	`Saccharopine dehydrogenase (By similarity).`

Sequence information

Length: 926 AA [This is the length of the unprocessed precursor]

Molecular weight: 102084 Da [This is the MW of the unprocessed precursor]

CRC64: 81A826B1856D9604 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLRVSRTKLG RLSPSLSRGL HHKAVMALRR EDVNAWERRA PLAPRHVKGI TNLGYKVLIQ 

        70         80         90        100        110        120 
PSNRRAIHDK EYVKAGGILQ EDISEACLIL GVKRPPEEKL MPKKTYAFFS HTIKAQEANM 

       130        140        150        160        170        180 
GLLDEILKQE IRLIDYEKMV DHRGIRVVAF GQWAGVAGII NILHGMGLRL LALGHHTPFM 

       190        200        210        220        230        240 
HIGMAHNYRN SGQAVQAVRD AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQEIFNELPC 

       250        260        270        280        290        300 
EYVEPHELKE VSQNGDLRKV YGTVLSRHHH LVRKTDGVYD PVEYDKYPER YISRFNTDIA 

       310        320        330        340        350        360 
PYTTCLINGI YWEQNTPRLL TRQDAQSLLA PGKSPVAGVE GCPALPHKLV AICDISADTG 

       370        380        390        400        410        420 
GSIEFMTECT TIERPFCMYD ADQHIIHDSV EGSGILMCSI DNLPAQLPIE STEYFGDMLY 

       430        440        450        460        470        480 
PYVEEMILSD ATQPLESQNF SPVVRDAVIA SNGMLSNKYK YIQKLRENRE HAQSLSMGTK 

       490        500        510        520        530        540 
KKVLVLGSGY VSEPVLEYLL RDDSIEITVG SDMKNQIEQL GKKYNINPVS LHVGKQEEKL 

       550        560        570        580        590        600 
SSLVATQDLV ISLLPYVLHP LVAKACIASK VNMITASYIT PALKELEKSV EDAGITVIGE 

       610        620        630        640        650        660 
LGLDPGLDHM LAMETIDKAK EVGATIESYV SYCGGLPAPE CSDNPLRYKF SWSPVGVLMN 

       670        680        690        700        710        720 
IMQPATYLLN GKVVNAVGGV SFLDSVTPMD YFPGLNLEGY PNRDSTKYAE IYGIPSAHTL 

       730        740        750        760        770        780 
LRGTLRYKGY AKALSGFVKL GLINRDAFPA LQPDANPLTW KELLCDLVGI SSSSKCDVLK 

       790        800        810        820        830        840 
EAVFKKLGGD TTQLEALEWL GLLGDEQVPQ AESLVDALSK HLAVKLSYGP GEKDMIVMRD 

       850        860        870        880        890        900 
SFGIRHPSGH LENKTIDLVV YGDVNGFSAM AKTVGLPTAM AAKMLLDGEI QAKGLMGPFS 

       910        920 
KEIYGPILER IKAEGIMYTT QSTIKL

A8E657 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools