FUNCTION: Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG (By similarity).
CATALYTIC ACTIVITY: (S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate.
CATALYTIC ACTIVITY: 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Copyright

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Cross-references

Sequence databases

EMBL

CAAC02000450; CAP23809.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

A8WTJ7.

Organism-specific databases

WormBase

WBGene00025759; CBG02769.

Ontologies

GO:0005739;	Cellular component: mitochondrion (inferred from sequence or structural similarity from UniProtKB).
GO:0047988;	Molecular function: hydroxyacid-oxoacid transhydrogenase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0015993;	Biological process: molecular hydrogen transport (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR001670; Fe_AlcDHase.
Graphical view of domain structure.

Pfam

PF00465; Fe-ADH; 1.
Pfam graphical view of domain structure.

PROSITE

PS00913; ADH_IRON_1; 1.
PS00060; ADH_IRON_2; FALSE_NEG.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Mitochondrion; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Mitochondrion (Potential).`
`CHAIN`	`? 465`		`Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial.`	`PRO_0000324755`

Sequence information

Length: 465 AA [This is the length of the unprocessed precursor]

Molecular weight: 50702 Da [This is the MW of the unprocessed precursor]

CRC64: 3B9DA79103CD2DE6 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSASLARGML HKMGGSCCPH HAPTTNPFRM AKLHGNNKST DYAFEMVCST LRFGKGVTLE 

        70         80         90        100        110        120 
IGYDVRNLGA KKTLLITDKN VQNTIAFKNA EQALKMVNIE YEIFDDVLIE PSEKSMQKAI 

       130        140        150        160        170        180 
AFAKSKPFDS FIAVGGGSVI DTTKAAALYA SNPEADFLDF VGPPFGKSLQ PKNPMLPLIA 

       190        200        210        220        230        240 
VPTTAGTGSE TTAAAIMDLP EHKCKTGIRL RCIKPYLAVV DPLNVMSMPR NVAIYSGFDV 

       250        260        270        280        290        300 
LCHALESYTA LPYDQRSPRP ARPEVRPVYQ GSNPISDVWS REALRIIGQY FRRSVFDPSD 

       310        320        330        340        350        360 
EEARTEMLKA SSFAGIGFGN AGVHLCHGLS YPISSQAKGC VAHDYPQDKN LIPHGLSVMT 

       370        380        390        400        410        420 
TAVADFEFTT AACPDRHLVA AQTLGADIPN NADNEYISRT LCDQLRGFMK DFGVPNGLKG 

       430        440        450        460 
MGFEYSDIDH LTEAASHSVP NIVISPKSTD RDIISKLYEK SLTVY

A8WTJ7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools