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UniProtKB/Swiss-Prot entry O27784


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MER_METTH
Primary accession number O27784
Secondary accession number Q50540
Integrated into Swiss-Prot on November 28, 2003
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 58)
Name and origin of the protein
Protein name 5,10-methylenetetrahydromethanopterin reductase
Synonyms EC 1.5.99.11
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase
Gene name
Name: mer
OrderedLocusNames: MTH_1752
From
Methanobacterium thermoautotrophicum [TaxID: 187420] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Delta H;
PubMed=8522533 [NCBI, ExPASy, EBI, Israel, Japan]
Noelling J., Pihl T.D., Reeve J.N.;
"Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri.";
J. Bacteriol. 177:7238-7244(1995).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Delta H;
PubMed=9371463 [NCBI, ExPASy, EBI, Israel, Japan]
Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J., Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D., Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R., Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D., Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A., Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J., Reeve J.N.;
"Complete genome sequence of Methanobacterium thermoautotrophicum deltaH: functional analysis and comparative genomics.";
J. Bacteriol. 179:7135-7155(1997).
[3]
CHARACTERIZATION.
STRAIN=Delta H;
PubMed=2298726 [NCBI, ExPASy, EBI, Israel, Japan]
te Broemmelstroet B.W., Hensgens C.M.H., Keltjens J.T., van der Drift C., Vogels G.D.;
"Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H.";
J. Biol. Chem. 265:1852-1857(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U31568; AAA92087.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE000666; AAB86222.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D69101; D69101.
RefSeq NP_276862.1; -.
3D structure databases
HSSP Q50744; 1F07. [HSSP ENTRY / PDB]
SMR O27784; 1-321.
ModBase O27784.
Enzyme and pathway databases
BioCyc MetaCyc:MERMAUTO-MON; -.
MTHE187420:MTH1752-MON; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0018537; Molecular function: coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_01091; -; 1.
PBIL [Tree]
InterPro IPR011251; Luciferase-like_bac.
IPR016048; Luciferase_mOase.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.30; Luciferase_like; 1.
Pfam PF00296; Bac_luciferase; 1.
Pfam graphical view of domain structure.
BLOCKS O27784.
Genome annotation databases
GeneID 1470837; -.
GenomeReviews AE000666_GR; MTH_1752.
KEGG mth:MTH1752; -.
NMPDR fig|187420.1.peg.1717; -.
Phylogenomic databases
HOGENOM O27784; -.
Genome annotation databases
CMR O27784; MTH_1752.
Other
ProtoNet O27784.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Methanogenesis; One-carbon metabolism; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   321  321     5,10-methylenetetrahydromethanopterin reductase. PRO_0000084810
CONFLICT   313   313        L -> H (in Ref. 1; AAA92087). 
Sequence information
Length: 321 AA [This is the length of the unprocessed precursor] Molecular weight: 33479 Da [This is the MW of the unprocessed precursor] CRC64: F1E7B93500E3C852 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKFGIEFVPN EPIEKIVKLV KLAEDVGFEY AWITDHYNNK NVYETLALIA EGTETIKLGP 

        70         80         90        100        110        120 
GVTNPYVRSP AITASAIATL DELSNGRATL GIGPGDKATF DALGIEWVKP VSTIRDAIAM 

       130        140        150        160        170        180 
MRTLLAGEKT ESGAQLMGVK AVQEKIPIYM GAQGPMMLKT AGEISDGALI NASNPKDFEA 

       190        200        210        220        230        240 
AVPLIKEGAE SAGKSLSDID VAAYTCCSID EDSAAAANAA KIVVAFIAAG SPPPVFERHG 

       250        260        270        280        290        300 
LPADTGAKFG ELLGKGDFGG AIGAVDDALM EAFSVVGTPD EFIPKIEALG EMGVTQYVAG 

       310        320 
SPIGPDKEKS IKLLGEVIAS F 

O27784 in FASTA format

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