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UniProtKB/Swiss-Prot entry O35728


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CP4AE_MOUSE
Primary accession number O35728
Secondary accession numbers A2A975 Q3UER2 Q5EBH0
Integrated into Swiss-Prot on February 7, 2006
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 58)
Name and origin of the protein
Protein name Cytochrome P450 4A14 [Precursor]
Synonyms EC 1.14.15.3
CYPIVA14
Lauric acid omega-hydroxylase 3
P450-LA-omega 3
Gene name
Name: Cyp4a14
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION.
STRAIN=C57BL/6 X CBA;
PubMed=9271096 [NCBI, ExPASy, EBI, Israel, Japan]
Heng Y.M., Kuo C.-W.S., Jones P.S., Savory R., Shultz R.M., Tomlinson S.R., Gray T.J.B., Bell D.R.;
"A novel murine P-450 gene, Cyp4a14, is part of a cluster of Cyp4a and Cyp4b, but not of CYP4F, genes in mouse and humans.";
Biochem. J. 325:741-749(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Kidney, and Liver;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=C57BL/6J;
The mouse genome sequencing consortium;
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Kidney;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
Comments
  • FUNCTION: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • CATALYTIC ACTIVITY: Octane + reduced rubredoxin + O2 = 1-octanol + oxidized rubredoxin + H2O.
  • COFACTOR: Heme group (By similarity).
  • SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • TISSUE SPECIFICITY: Very low level in liver, kidney and spleen.
  • INDUCTION: By peroxisome proliferator methylclofenapate; 1000-fold in liver, 10-fold in kidney.
  • SIMILARITY: Belongs to the cytochrome P450 family.
  • SEQUENCE CAUTION:
    • Sequence=BC089609; Type=Erroneous termination; Positions=365; Note=Translated as Gln
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Y11638; CAA72345.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y11639; CAA72345.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y11640; CAA72345.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y11641; CAA72345.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Y11642; CAA72345.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK149392; BAE28849.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK165452; BAE38193.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL627182; CAM21089.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC089609; -; NOT_ANNOTATED_CDS; mRNA.[EMBL / GenBank / DDBJ]
RefSeq NP_031848.1; -.
UniGene Mm.250901
3D structure databases
ModBase O35728.
Organism-specific databases
MGI MGI:1096550; Cyp4a14.
Gene expression databases
ArrayExpress O35728; -.
GermOnline ENSMUSG00000028715; Mus musculus.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS O35728.
Genome annotation databases
Ensembl ENSMUSG00000028715; Mus musculus. [Contig view]
GeneID 13119; -.
KEGG mmu:13119; -.
Phylogenomic databases
HOGENOM O35728; -.
HOVERGEN O35728; -.
Other
SOURCE Cyp4a14; Mus musculus.
ProtoNet O35728.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
PROPEP   1     4  4     Removed in mature form (By similarity). PRO_0000046058
CHAIN   5   507  503     Cytochrome P450 4A14. PRO_0000046059
METAL   454   454        Iron (heme axial ligand) (By similarity). 
BINDING   318   318        Heme (covalent; via 1 link) (By similarity). 
MOD_RES   437   437        Phosphoserine (By similarity). 
CONFLICT   231   231        N -> D (in Ref. 2; BAE28849). 
CONFLICT   278   278        Q -> R (in Ref. 4; BC089609). 
CONFLICT   401   401        S -> F (in Ref. 4; BC089609). 
Sequence information
Length: 507 AA [This is the length of the unprocessed precursor] Molecular weight: 58720 Da [This is the MW of the unprocessed precursor] CRC64: EB46205838D4549C [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGFFLFSPTR YLDGISGFFQ WAFLLSLFLV LFKAVQFYLR RQWLLKTLQH FPCMPSHWLW 

        70         80         90        100        110        120 
GHHLKDKELQ QILIWVEKFP SACLQCLSGS NIRVLLYDPD YVKVVLGRSD PKASGIYQFF 

       130        140        150        160        170        180 
APWIGYGLLL LNGKKWFQHR RMLTPAFHYD ILKPYVKIMA DSVNIMLDKW EKLDGQDHPL 

       190        200        210        220        230        240 
EIFHCVSLMT LDTVMKCAFS YQGSVQLDEN SKLYTKAVED LNNLTFFRLR NAFYKYNIIY 

       250        260        270        280        290        300 
NMSSDGRLSH HACQIAHEHT DGVIKMRKSQ LQNEEELQKA RKKRHLDFLD ILLFARMEDR 

       310        320        330        340        350        360 
NSLSDEDLRA EVDTFMFEGH DTTASGISWI FYALATHPEH QQRCREEVQS ILGDGTSVTW 

       370        380        390        400        410        420 
DHLGQMPYTT MCIKEALRLY PPVISVSREL SSPVTFPDGR SIPKGITATI SIYGLHHNPR 

       430        440        450        460        470        480 
FWPNPKVFDP SRFAPDSSHH SHAYLPFSGG SRNCIGKQFA MNELKVAVAL TLLRFELLPD 

       490        500 
PTRIPVPIAR LVLKSKNGIH LCLKKLR 

O35728 in FASTA format

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