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UniProtKB/Swiss-Prot entry O43007


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name C1TM_SCHPO
Primary accession number O43007
Secondary accession numbers None
Integrated into Swiss-Prot on January 15, 2008
Sequence was last modified on November 1, 1999 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 42)
Name and origin of the protein
Protein name C-1-tetrahydrofolate synthase, mitochondrial [Precursor]
Synonym C1-THF synthase
Includes Methylenetetrahydrofolate dehydrogenase
     (EC 1.5.1.5)
Methenyltetrahydrofolate cyclohydrolase
     (EC 3.5.4.9)
Formyltetrahydrofolate synthetase
     (EC 6.3.4.3)
Gene name
Name: ade9
ORFNames: SPBC2G2.08
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[2]
SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
DOI=10.1038/nbt1222; PubMed=16823372 [NCBI, ExPASy, EBI, Israel, Japan]
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.;
"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.";
Nat. Biotechnol. 24:841-847(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
CU329671; CAA17888.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T40147; T40147.
RefSeq NP_596437.1; -.
3D structure databases
HSSP P11586; 1A4I. [HSSP ENTRY / PDB]
ModBase O43007.
Organism-specific databases
GeneDB_Spombe SPBC2G2.08; -.
Gene expression databases
ArrayExpress O43007; -.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR000559; Fmtethyd_synth.
IPR016040; NAD(P)-bd.
IPR000672; THF_DHase/CycOHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF01268; FTHFS; 1.
PF00763; THF_DHG_CYH; 1.
PF02882; THF_DHG_CYH_C; 1.
Pfam graphical view of domain structure.
PRINTS PR00085; THFDHDRGNASE.
ProDom PD002300; THFDhg/Cyc_hydro; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00721; FTHFS_1; 1.
PS00767; THF_DHG_CYH_2; 1.
BLOCKS O43007.
Genome annotation databases
GeneID 2540300; -.
KEGG spo:SPBC2G2.08; -.
NMPDR fig|4896.1.peg.2303; -.
Other
ProtoNet O43007.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amino-acid biosynthesis; ATP-binding; Complete proteome; Histidine biosynthesis; Hydrolase; Ligase; Methionine biosynthesis; Mitochondrion; Multifunctional enzyme; NADP; Nucleotide-binding; One-carbon metabolism; Oxidoreductase; Purine biosynthesis; Transit peptide.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
TRANSIT   1    52  52     Mitochondrion (Potential). 
CHAIN   53   969  917     C-1-tetrahydrofolate synthase, mitochondrial. PRO_0000315627
NP_BIND   199   201  3     NADP (By similarity). 
NP_BIND   402   409  8     ATP (By similarity). 
REGION   53   337  285     Methylenetetrahydrofolate dehydrogenase and cyclohydrolase. 
REGION   81    85  5     Substrate binding (By similarity). 
REGION   128   130  3     Substrate binding (By similarity). 
REGION   296   300  5     Substrate binding (By similarity). 
REGION   338   969  632     Formyltetrahydrofolate synthetase. 
BINDING   224   224        NADP (By similarity). 
Sequence information
Length: 969 AA [This is the length of the unprocessed precursor] Molecular weight: 105412 Da [This is the MW of the unprocessed precursor] CRC64: DC8C3FF5B14D4C68 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVSFNQLRNY FLESNSLRPS KWLFQSYGTS SSANILNGKL LARKLQRSVA EEVQALKAKD 

        70         80         90        100        110        120 
RNFKPALAIV QVGKREDSNV YVRMKEKAAR LVGIDFKYCP FPETIQMPAL LHELKKLNDD 

       130        140        150        160        170        180 
HTVHGVLVQL PLPKHLNERT VTESITPPKD VDGFGAFNIG LLAKNDATPI HYPCTPKGIM 

       190        200        210        220        230        240 
ELLKDNKISV AGLNAVVLGR SDIVGNPISY LLRKDNATVT VCHSKTKDLI QHISNADLVI 

       250        260        270        280        290        300 
AALGKPEFVR GEWLKPGSVV VDVGINAVQR NGKRVLVGDV HFESASKVAS SITPVPGGVG 

       310        320        330        340        350        360 
PMTVAMLMEN IVNAAKIART ENIYRKIDLN PLELKKPVPS DIEIANSQEP KLISNLAKEM 

       370        380        390        400        410        420 
GIYDTELENY GNYKAKVNLA VYERLKHRKD GNYVVVSGIT PTPFGEGKST VVAGLVQAMG 

       430        440        450        460        470        480 
HLGKLGIACV RQPSQGPTFG VKGGAAGGGY AQFIPMDDFN LHMTGDIHAV TAANNLLVAA 

       490        500        510        520        530        540 
LETRMFHENT QSDAALIKRL IPVKNGRRVI PRGLIGRWNR ICASHNMDPE DVNNASPELL 

       550        560        570        580        590        600 
KEFVRLNVDP DTIECNRVLD VNDRFLRSIE VGKASTEKGH VRKTSFDISV ASECMSILAL 

       610        620        630        640        650        660 
SCDLNDMHSR LSRMVIANDK YGNAITAGDL GVSGALTVLL KDAIKPNLMQ TLEGTPAFVH 

       670        680        690        700        710        720 
AGPFANISIG ASSIIADKIA LKLAGTESFD RPEDAGYVVT EAGFASDMGM EKFFNIKCRY 

       730        740        750        760        770        780 
SKLVPNTVVL VTTVKALKLH GGGPKLKPGA PIPEEYLVEN LDLVKNGCSN MVKHIQNCHK 

       790        800        810        820        830        840 
FNIPVVVAIN SYKTDSSKEH EIIREAALQA GAVDAVPSDH WAQGGKGAIE LAKSVMTACD 

       850        860        870        880        890        900 
QSSNSKFRLL YDSETSIEDK VNVIAKEMYG ANGVEFSSLA KERINTFIKQ GFGNLPICMA 

       910        920        930        940        950        960 
KTQYSLSHNP EFRNVPKNFT VPIRDMRLNA GAGFIYPLAA EIQTIPGLPT APAYLNIDIC 


ENGEIVGLS 

O43007 in FASTA format

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