NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. SS1000;
TISSUE=Etiolated seedling;
DOI=10.1023/A:1005915507497; PubMed=9484480 [NCBI, ExPASy, EBI, Israel, Japan]
Bak S., Kahn R.A., Nielsen H.L., Moeller B.L., Halkier B.A.;
"Cloning of three A-type cytochromes P450, CYP71E1, CYP98, and CYP99 from Sorghum bicolor (L.) Moench by a PCR approach and identification by expression in Escherichia coli of CYP71E1 as a multifunctional cytochrome P450 in the biosynthesis of the cyanogenic glucoside dhurrin.";
Plant Mol. Biol. 36:393-405(1998).

Comments

FUNCTION: Catalyzes the conversion of p-hydroxyphenylacetaldoxime to p-hydroxymandelonitrile. The dehydration of the oxime to the corresponding nitrile is followed by a C-hydroxylation of the nitrile to produce p-hydroxymandelonitrile.
CATALYTIC ACTIVITY: (Z)-4-hydroxyphenylacetaldehyde oxime + NADPH + O₂ = (S)-4-hydroxymandelonitrile + NADP⁺ + 2 H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Secondary metabolite biosynthesis; dhurrin biosynthesis; dhurrin from L-tyrosine: step 2/3 .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein (Potential).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF029858; AAC39318.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T14640; T14640.

3D structure databases

HSSP

P00179; 1DT6. [HSSP ENTRY / PDB]

ModBase

O48958.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-522; -.

Organism-specific databases

Gramene

O48958; -.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 531`	`531`	`4-hydroxyphenylacetaldehyde oxime monooxygenase.`	`PRO_0000052123`
`TRANSMEM`	`18 38`	`21`	`Potential.`
`METAL`	`468 468`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 531 AA [This is the length of the unprocessed precursor]

Molecular weight: 59088 Da [This is the MW of the unprocessed precursor]

CRC64: 2272E4AA910890D2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATTATPQLL GGSVPQQWQT CLLVLLPVLL VSYYLLTSRS RNRSRSGKLG GAPRLPPGPA 

        70         80         90        100        110        120 
QLPILGNLHL LGPLPHKNLR ELARRYGPVM QLRLGTVPTV VVSSAEAARE VLKVHDVDCC 

       130        140        150        160        170        180 
SRPASPGPKR LSYDLKNVGF APYGEYWREM RKLFALELLS MRRVKAACYA REQEMDRLVA 

       190        200        210        220        230        240 
DLDRAAASKA SIVLNDHVFA LTDGIIGTVA FGNIYASKQF AHKERFQHVL DDAMDMMASF 

       250        260        270        280        290        300 
SAEDFFPNAA GRLADRLSGF LARRERIFNE LDVFFEKVID QHMDPARPVP DNGGDLVDVL 

       310        320        330        340        350        360 
INLCKEHDGT LRFTRDHVKA IVLDTFIGAI DTSSVTILWA MSELMRKPQV LRKAQAEVRA 

       370        380        390        400        410        420 
AVGDDKPRVN SEDAAKIPYL KMVVKETLRL HPPATLLVPR ETMRDTTICG YDVPANTRVF 

       430        440        450        460        470        480 
VNAWAIGRDP ASWPAPDEFN PDRFVGSDVD YYGSHFELIP FGAGRRICPG LTMGETNVTF 

       490        500        510        520        530 
TLANLLYCYD WALPGAMKPE DVSMEETGAL TFHRKTPLVV VPTKYKNRRA A

O48958 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools