NUCLEOTIDE SEQUENCE [MRNA].
PubMed=9681018 [NCBI, ExPASy, EBI, Israel, Japan]
Pauli H.H., Kutchan T.M.;
"Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis.";
Plant J. 13:793-801(1998).

Comments

FUNCTION: 3'-hydroxylation of (S)-N-methylcoclaurine.
CATALYTIC ACTIVITY: (S)-N-methylcoclaurine + NADPH + O₂ = (S)-3'-hydroxy-N-methylcoclaurine + NADP⁺ + H₂O.
COFACTOR: Heme group.
PATHWAY: Alkaloid biosynthesis; (S)-reticuline biosynthesis; (S)-reticuline from (S)-norcoclaurine: step 3/4 .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass membrane protein (Potential). Microsome membrane; Single-pass membrane protein (Potential).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF014800; AAC39452.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T07960; T07960.

3D structure databases

HSSP

P00179; 1DT6. [HSSP ENTRY / PDB]

ModBase

O64899.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0050593;	Molecular function: N-methylcoclaurine 3'-monooxygenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 487`	`>487`	`(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1.`	`PRO_0000052157`
`TRANSMEM`	`4 24`	`21`	`Potential.`
`METAL`	`429 429`		`Iron (heme axial ligand) (By similarity).`
`NON_TER`	`1 1`

Sequence information

Length: 487 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 54644 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 2EF90A9B67500AE2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
GTSTVALIAV IISSILYLLF GGSGHKNLPP GPKPWPIVGN LLQLGEKPHA QFAELAQTYG 

        70         80         90        100        110        120 
DIFTLKMGTE TVVVASTSSA ASEILKTHDR ILSARYVFQS FRVKGHVENS IVWSDCTETW 

       130        140        150        160        170        180 
KNLRKVCRTE LFTQKMIESQ AHVREKKCEE MVEYLMKKQG EEVKIVEVIF GTLVNIFGNL 

       190        200        210        220        230        240 
IFSQNIFELG DPNSGSSEFK EYLWRMLELG NSTNPADYFP MLGKFDLFGQ RKEVAECLKG 

       250        260        270        280        290        300 
IYAIWGAMLQ ERKLAKKVDG YKSKNDFVDV CLDSGLNDYQ INALLMELFG AGTETSASTI 

       310        320        330        340        350        360 
EWAMTELTKN PKITAKIRSE IQTVVGERSV KESDFPNLPY LEATVKETLR LHPPTPLLLP 

       370        380        390        400        410        420 
RRALETCTIL NYTIPKDCQI MVNAWGIGRD PKTWTDPLTF SPERFLNSSV DFRGNDFSLI 

       430        440        450        460        470        480 
PFGAGRRICP GLPIANQFIA LLVATFVQNL DWCLPNGMSV DHLIVEEKFG LTLQKEPPLF 


IVPKSRV

O64899 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools