NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1007/s002030050658; PubMed=9818358 [NCBI, ExPASy, EBI, Israel, Japan]
Hochheimer A., Hedderich R., Thauer R.K.;
"The formylmethanofuran dehydrogenase isoenzymes in Methanobacterium wolfei and Methanobacterium thermoautotrophicum: induction of the molybdenum isoenzyme by molybdate and constitutive synthesis of the tungsten isoenzyme.";
Arch. Microbiol. 170:389-393(1998).

Comments

FUNCTION: Catalyzes the reversible oxidation of CO(2) and methanofuran (MFR) to N-formylmethanofuran (CHO-MFR). This enzyme is oxygen-labile (By similarity).
CATALYTIC ACTIVITY: Formylmethanofuran + H₂O + acceptor = CO₂ + methanofuran + reduced acceptor.
COFACTOR: Tungsten.
PATHWAY: One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methenyl-H(4)MPT from CO(2): step 1/3 .
SUBUNIT: This enzyme is composed of six subunits fwdA, fwdB, fwdC, fwdD, fwdF and fwdG (By similarity).
INDUCTION: By growth on tungsten or molybdenum under anaerobic conditions.
SIMILARITY: Belongs to the fwdC/fmdC family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ009688; CAA08784.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

O74031.

Family and domain databases

InterPro

IPR017550; Formylmethanofuran_DH_C.
IPR002489; Glu_synthase_C.
Graphical view of domain structure.

Gene3D

G3DSA:2.160.20.60; Glu_synthase_C; 1.

Pfam

PF01493; GXGXG; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methanogenesis; Oxidoreductase; Repeat; Tungsten.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 270`	`270`	`Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C.`	`PRO_0000144198`
`REPEAT`	`80 92`	`13`	`1.`
`REPEAT`	`99 111`	`13`	`2.`
`REPEAT`	`118 130`	`13`	`3.`
`REPEAT`	`144 156`	`13`	`4.`
`REPEAT`	`163 175`	`13`	`5.`
`REPEAT`	`182 194`	`13`	`6.`
`REPEAT`	`201 213`	`13`	`7.`
`REGION`	`80 213`	`134`	`7 X 13 AA repeats of [GW]-X-X-M-X-X-G-X-[IL]-X-[IV]-X-G.`

Sequence information

Length: 270 AA [This is the length of the unprocessed precursor]

Molecular weight: 28660 Da [This is the MW of the unprocessed precursor]

CRC64: BB1061CBDF53061B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSEIILTPKE QPEVPLEAPN IKPDVFAGKS IDEIKNIQIM HGNEVVKLGD FFEVSGEPAD 

        70         80         90        100        110        120 
APEDIKIIID GDVYNTKRIG QEMTAGEIIV RGNVNMYVGA GMKGGKITVE GNAGSWAGQD 

       130        140        150        160        170        180 
MRGGEIEILG DADDYVGSSY RGDWRGMSGG TITVHGNADN EIGEYMNGGK IIIKGDVNIM 

       190        200        210        220        230        240 
PGIHMNNGLI IIEGNVVARA GGEMAGGTIV VKGMMQEFLA GFKYLGVEKD IELMVKNSPG 

       250        260        270 
AFYKFEGDHA IKGAKGIVYA AVGCNGHIAP

O74031 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools