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UniProtKB/Swiss-Prot entry O74927


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MTHR2_SCHPO
Primary accession number O74927
Secondary accession numbers P78770 Q9UT80
Integrated into Swiss-Prot on July 15, 1999
Sequence was last modified on August 14, 2001 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 54)
Name and origin of the protein
Protein name Methylenetetrahydrofolate reductase 2
Synonym EC 1.5.1.20
Gene name
Name: met11
Synonyms: mthfr2
ORFNames: SPAC343.10
From
Schizosaccharomyces pombe (Fission yeast) [TaxID: 4896] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 38364 / 968;
Naula N.;
Thesis (1998), University of Bern, Switzerland.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 38366 / 972;
DOI=10.1038/nature724; PubMed=11859360 [NCBI, ExPASy, EBI, Israel, Japan]
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
"The genome sequence of Schizosaccharomyces pombe.";
Nature 415:871-880(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-641.
STRAIN=PR745;
DOI=10.1093/dnares/4.6.363; PubMed=9501991 [NCBI, ExPASy, EBI, Israel, Japan]
Yoshioka S., Kato K., Nakai K., Okayama H., Nojima H.;
"Identification of open reading frames in Schizosaccharomyces pombe cDNAs.";
DNA Res. 4:363-369(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AJ011686; CAA09738.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
CU329670; CAB52273.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D89118; BAA13780.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T38659; T38659.
RefSeq NP_593430.1; -.
3D structure databases
HSSP P00394; 1B5T. [HSSP ENTRY / PDB]
ModBase O74927.
Enzyme and pathway databases
BioCyc SPOM-XXX-01:SPOM-XXX-01-001116-MON; -.
Organism-specific databases
GeneDB_Spombe SPAC343.10; -.
Gene expression databases
ArrayExpress O74927; -.
Ontologies
GO
GO:0004489; Molecular function: methylenetetrahydrofolate reductase (NADPH) activity (inferred from direct assay from GeneDB_SPombe).
GO:0006555; Biological process: methionine metabolic process (inferred from direct assay from GeneDB_SPombe).
QuickGo view.
Family and domain databases
InterPro IPR004621; Fadh2_euk.
IPR003171; Mehydrof_redctse.
Graphical view of domain structure.
Pfam PF02219; MTHFR; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00677; fadh2_euk; 1.
BLOCKS O74927.
Genome annotation databases
GeneID 2543105; -.
KEGG spo:SPAC343.10; -.
NMPDR fig|4896.1.peg.3400; -.
Other
ProtoNet O74927.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; FAD; Flavoprotein; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   641  641     Methylenetetrahydrofolate reductase 2. PRO_0000190254
CONFLICT   202   202        E -> D (in Ref. 3; BAA13780). 
CONFLICT   281   281        G -> A (in Ref. 3; BAA13780). 
CONFLICT   455   455        A -> V (in Ref. 3; BAA13780). 
CONFLICT   537   641        QVTYYAGNNKSEFLTNAPKDGASAVTWGVYPGREIIQSTI IAEVSFKAWLSESFQVWGEWANLYSKNTPSRKLLENCIND RWLVTVIHHDFMDKNGLWKVLEIDF -> HVRSNFSSSISDSTCFQRLVSKPLFLRIRATDRSKFSVQK (in Ref. 3; BAA13780). 
CONFLICT   614   614        I -> L (in Ref. 1; CAA09738). 
Sequence information
Length: 641 AA [This is the length of the unprocessed precursor] Molecular weight: 72140 Da [This is the MW of the unprocessed precursor] CRC64: E7AFFDD1F2C1C8A4 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKIVDLIDKI PNGNAFYSFE YFPPKTTVGL ENLSSRITRM SRNMMPLFSS VTWGTAGSTA 

        70         80         90        100        110        120 
EVSIYLAKML EQHHKIPACL HLTCTNVDKA IIDKALETAK EYGIRNILAL RGDPPLNSDH 

       130        140        150        160        170        180 
WEGKVSEFEH AVDLVRYIRE KYGDYFCIGV AAYPEGHVDS NVPELSKDPL RDIPFLIEKV 

       190        200        210        220        230        240 
EAGADFIITQ IFYEPEAFIK FENFVRNHSS NALRNIPIIP AIMPIQSYGS LKRMTRLCGC 

       250        260        270        280        290        300 
SVPSSLMQRL NAAKPDDEAI KNIGVEHIVD MIKKIMDNVQ GRVHGFHFCT LNLERSVALI 

       310        320        330        340        350        360 
LKNSGLLTKR WKQVESEMED EKLLRTTRKR LSLDEPAELH NQVVVPSQQP VADKSSNLFV 

       370        380        390        400        410        420 
TSKQSSVSGH KDNLTEEAPF SVSEGSGVLG RQANWDDFTN GRFGDPRSPA YGEIDGYGPT 

       430        440        450        460        470        480 
LHFPPSEALK LWGYPVDESD ITSLFQKHIM SDISAIPWID EPVEVETKTI AKYLLKLNGN 

       490        500        510        520        530        540 
SWWTVGSQPA VNGAPSADPV FGWGPKGGRV FQKAFVECFV NGKDLKDFIT KWHDNPQVTY 

       550        560        570        580        590        600 
YAGNNKSEFL TNAPKDGASA VTWGVYPGRE IIQSTIIAEV SFKAWLSESF QVWGEWANLY 

       610        620        630        640 
SKNTPSRKLL ENCINDRWLV TVIHHDFMDK NGLWKVLEID F 

O74927 in FASTA format

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