NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-20, AND CHARACTERIZATION.
PubMed=9721273 [NCBI, ExPASy, EBI, Israel, Japan]
Achterholt S., Priefert H., Steinbuechel A.;
"Purification and characterization of the coniferyl aldehyde dehydrogenase from Pseudomonas sp. strain HR199 and molecular characterization of the gene.";
J. Bacteriol. 180:4387-4391(1998).

Comments

FUNCTION: Catalyzes the NAD+-dependent oxidation of coniferyl aldehyde to ferulic acid and which is induced during growth with eugenol as the carbon source.
CATALYTIC ACTIVITY: Coniferyl aldehyde + H₂O + NAD(P)⁺ = ferulate + NAD(P)H.
SUBUNIT: Homodimer.
MISCELLANEOUS: The enzyme activity with NADP(+) is 4.3% of that with NAD(+).
SIMILARITY: Belongs to the aldehyde dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ006231; CAA06926.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P11883; 1AD3. [HSSP ENTRY / PDB]

ModBase

O86447.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR012394; Ald_DHase_NAD(P).
IPR015590; Aldehyde_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11699; Aldehyde_dehyd; 1.
PTHR11699:SF15; ALDH; 1.

Pfam

PF00171; Aldedh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF036492; ALDH; 1.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; FALSE_NEG.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed.`
`CHAIN`	`2 481`	`480`	`Coniferyl aldehyde dehydrogenase.`	`PRO_0000056585`
`ACT_SITE`	`221 221`		`By similarity.`
`ACT_SITE`	`255 255`		`By similarity.`

Sequence information

Length: 481 AA [This is the length of the unprocessed precursor]

Molecular weight: 51987 Da [This is the MW of the unprocessed precursor]

CRC64: A1AC6CC1CB1D55B1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSILGLNGAP VGAEQLGSAL DRMKKAHLEQ GPANLELRLS RLDRAIAMLL ENREAIADAV 

        70         80         90        100        110        120 
SADFGNRSRE QTLLCDIAGS VASLKDSREH VAKWMEPEHH KAMFPGAEAR VEFQPLGVVG 

       130        140        150        160        170        180 
VISPWNFPIV LAFGPLAGIF AAGNRAMLKP SELTPRTSAL LAELIARYFD ETELTTVLGD 

       190        200        210        220        230        240 
AEVGALFSAQ PFDHLIFTGG TAVAKHIMRA AADNLVPVTL ELGGKSPVIV SRSADMADVA 

       250        260        270        280        290        300 
QRVLTVKTFN AGQICLAPDY VLLPEESLDS FVAEATRFVA AMYPSLLDNP DYTSIINARN 

       310        320        330        340        350        360 
FDRLHRYLTD AQAKGGRVIE INPAAEELGD SGIRKIAPTL IVNVSDEMLV LNEEIFGPLL 

       370        380        390        400        410        420 
PIKTYRDFDS AIDYVNSKQR PLASYFFGED AVEREQVLKR TVSGAVVVND VMSHVMMDTL 

       430        440        450        460        470        480 
PFGGVGHSGM GAYHGIYGFR TFSHAKPVLV QSPVGESNLA MRAPYGEAIH GLLSVLLSTE 


C

O86447 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools