ID   DPYD_RAT                Reviewed;        1025 AA.
AC   O89000;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   04-NOV-2008, entry version 53.
DE   RecName: Full=Dihydropyrimidine dehydrogenase [NADP+];
DE            Short=DHPDHase;
DE            Short=DPD;
DE            EC=1.3.1.2;
DE   AltName: Full=Dihydrouracil dehydrogenase;
DE   AltName: Full=Dihydrothymine dehydrogenase;
GN   Name=Dpyd; Synonyms=DPD;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Wistar; TISSUE=Liver;
RX   MEDLINE=99037149; PubMed=9819714;
RA   Kimura M., Fujimoto Sakata S., Matoba Y., Matsuda K., Kontani Y.,
RA   Kaneko M., Tamaki N.;
RT   "Cloning of rat dihydropyrimidine dehydrogenase and correlation of its
RT   mRNA increase in the rat liver with age.";
RL   J. Nutr. Sci. Vitaminol. 44:537-546(1998).
CC   -!- FUNCTION: Involved in pyrimidine base degradation. Catalyzes the
CC       reduction of uracil and thymine (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,6-dihydrouracil + NADP(+) = uracil + NADPH.
CC   -!- COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- COFACTOR: FMN (By similarity).
CC   -!- ENZYME REGULATION: Inactivated by 5-iodouracil (By similarity).
CC   -!- PATHWAY: Amino-acid biosynthesis; beta-alanine biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the dihydropyrimidine dehydrogenase family.
CC   -!- SIMILARITY: Contains 3 4Fe-4S ferredoxin-type domains.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; D85035; BAA33218.1; -; mRNA.
DR   RefSeq; NP_112289.1; -.
DR   UniGene; Rn.158382; -.
DR   HSSP; Q28943; 1H7X.
DR   SMR; O89000; 2-1017.
DR   Ensembl; ENSRNOG00000017105; Rattus norvegicus.
DR   GeneID; 81656; -.
DR   KEGG; rno:81656; -.
DR   RGD; 621218; Dpyd.
DR   HOVERGEN; O89000; -.
DR   NextBio; 615210; -.
DR   ArrayExpress; O89000; -.
DR   GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR   GO; GO:0017113; F:dihydropyrimidine dehydrogenase (NADP+) act...; IDA:UniProtKB.
DR   GO; GO:0050661; F:NADP binding; IDA:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR   GO; GO:0006214; P:thymidine catabolic process; ISS:UniProtKB.
DR   GO; GO:0006212; P:uracil catabolic process; ISS:UniProtKB.
DR   InterPro; IPR001450; 4Fe4S_Fe_S_bd.
DR   InterPro; IPR000759; Adrndx_reductase.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR005720; DHO_DHase_1_core.
DR   InterPro; IPR001295; Dihydroorotate_DHase_core.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR012285; Fum_reductase_C.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   InterPro; IPR000103; Pyridine_nuc-diS_OxRdtase_2.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   Gene3D; G3DSA:1.10.1060.10; Fum_reductase_C; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF01180; DHO_dh; 1.
DR   Pfam; PF00037; Fer4; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PRINTS; PR00353; 4FE4SFRDOXIN.
DR   PRINTS; PR00419; ADXRDTASE.
DR   PRINTS; PR00368; FADPNR.
DR   PRINTS; PR00469; PNDRDTASEII.
DR   ProDom; PD000139; FAD_pyr_redox; 1.
DR   TIGRFAMs; TIGR01037; pyrD_sub1_fam; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 3.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Cytoplasm; FAD; Flavoprotein; FMN; Iron; Iron-sulfur;
KW   Metal-binding; NADP; Oxidoreductase; Phosphoprotein; Repeat.
FT   CHAIN         1   1025       Dihydropyrimidine dehydrogenase [NADP+].
FT                                /FTId=PRO_0000327502.
FT   DOMAIN       69    100       4Fe-4S ferredoxin-type 1.
FT   DOMAIN      944    976       4Fe-4S ferredoxin-type 2.
FT   DOMAIN      978   1007       4Fe-4S ferredoxin-type 3.
FT   METAL       953    953       Iron-sulfur 1 (4Fe-4S) (Potential).
FT   METAL       956    956       Iron-sulfur 1 (4Fe-4S) (Potential).
FT   METAL       959    959       Iron-sulfur 1 (4Fe-4S) (Potential).
FT   METAL       963    963       Iron-sulfur 1 (4Fe-4S) (Potential).
FT   METAL       986    986       Iron-sulfur 2 (4Fe-4S) (Potential).
FT   METAL       989    989       Iron-sulfur 2 (4Fe-4S) (Potential).
FT   METAL       992    992       Iron-sulfur 2 (4Fe-4S) (Potential).
FT   METAL       996    996       Iron-sulfur 2 (4Fe-4S) (Potential).
FT   MOD_RES     577    577       Phosphoserine (By similarity).
SQ   SEQUENCE   1025 AA;  111468 MW;  07859E73D249828C CRC64;
     MAGVLSRDAP DIESILALNP RIQAHATLRS TMAKKLDKKH WKRNTDKNCF ICEKLENNFD
     DIKHTTLGER GALREAVRCL KCADAPCQKS CPTSLDIKSF ITSIANKNYY GAAKLIFSDN
     PLGLTCGMVC PTSDLCVGGC NLHATEEGPI NIGGLQQFAT EVFKAMNIPQ IRSPLLPPPE
     HMPEAYSAKI ALFGAGPASI SCASFLARLG YSDITIFEKQ EYVGGLSTSE IPQFRLPYDV
     VNFEIELMKD LGVKIICGKS ISTDEMTLST LKENGYKAAF IGIGLPEPKK DHIFQGLTQV
     QGFYTSKDFL PLVAKGSKPG MCACHSPLPS VRGAVIVLGA GDTAFDCATS ALRCGARRVF
     IVFRKGFANI RAVPEEMELA KEEKCEFLPF LSPRKVIVKD GKIVGMQFVR TEQDETGNWV
     EDEEQIVRLK ADVVISPFGS VLDDPKVIEA LSPIKFNRWG LPEVNPETMQ TSEPWVFAGG
     DVVGMANTTV ESVNDGKQAS WYIHEYIQAQ YGALVPSQPT LPLFYTPVDL VDISVEMAGL
     RFPNPFGLAS ATPATSTPMI RRAFEAGWGF ALTKTFSLDK DIVTNVSPRI IRGTTSGPLY
     GPGQSSFLNI ELISEKTAAY WCHSVTELKA DFPDNILIAS IMCSYNKNDW MELSKMAEAS
     GADALELNLS CPHGMGERGM GLACGQDPEL VRNICRWVRQ SVRVPFFAKL TPNVTDIVSI
     ARAAKEGGAD GVTATNTVSG LMGLKADGSP WPSVGSGKRT TYGGVSGTTI RPIALRAVTA
     IARALPGFPI LATGGIDSAE SGLQFLHSGA SVLQVCSAIQ NQDFTVIEDY CTGLKALLYL
     KSIEELSDWD GQSPPTMSHQ KGKPVPHIAE LMGQKLPSFG PYLERRKKIL AASKIRENDQ
     NRACSPLQRK HFNSQKPIPA IKDVIGKSLQ YLGTFGELNI MEQVVALIDE EMCINCGKCY
     MTCNDSGYQA IQFDPETHLP TVSDTCTGCT LCLSVCPIMD CIRMVSRATP YEPKRGLPLA
     VKPVC
//