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UniProtKB/Swiss-Prot entry P00369


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHE4_NEUCR
Primary accession number P00369
Secondary accession number Q7RVE1
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    September 2, 2008 (Entry version 76)
Name and origin of the protein
Protein name NADP-specific glutamate dehydrogenase
Synonyms NADP-GDH
EC 1.4.1.4
NADP-dependent glutamate dehydrogenase
Gene name
Name: gdh
Synonyms: am
ORFNames: 18F11.230, NCU01195
From
Neurospora crassa [TaxID: 5141] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(83)90195-6; PubMed=6231215 [NCBI, ExPASy, EBI, Israel, Japan]
Kinnaird J.H., Fincham J.R.S.;
"The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene.";
Gene 26:253-260(1983).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
DOI=10.1093/nar/gkg293; PubMed=12655011 [NCBI, ExPASy, EBI, Israel, Japan]
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence.";
Nucleic Acids Res. 31:1944-1954(2003).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
DOI=10.1038/nature01554; PubMed=12712197 [NCBI, ExPASy, EBI, Israel, Japan]
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
"The genome sequence of the filamentous fungus Neurospora crassa.";
Nature 422:859-868(2003).
[4]
PROTEIN SEQUENCE OF 2-454.
PubMed=1002 [NCBI, ExPASy, EBI, Israel, Japan]
Holder A.A., Wootton J.C., Baron A.J., Chambers G.K., Fincham J.R.S.;
"The amino acid sequence of Neurospora NADP-specific glutamate dehydrogenase. Peptic and chymotryptic peptides and the complete sequence.";
Biochem. J. 149:757-773(1975).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
DOI=10.1016/0378-1119(82)90207-4; PubMed=6299898 [NCBI, ExPASy, EBI, Israel, Japan]
Kinnaird J.H., Keighren M.A., Kinsey J.A., Eaton M., Fincham J.R.S.;
"Cloning of the am (glutamate dehydrogenase) gene of Neurospora crassa through the use of a synthetic DNA probe.";
Gene 20:387-396(1982).
[6]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-25.
DOI=10.1016/0378-1119(84)90242-7; PubMed=6325296 [NCBI, ExPASy, EBI, Israel, Japan]
Rambosek J.A., Kinsey J.A.;
"An unstable mutant gene of the am locus of Neurospora results from a small duplication.";
Gene 27:101-107(1984).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
K01409; AAA33558.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL670011; CAD21426.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AABX02000015; EAA32325.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K01653; AAA33557.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K02468; AAA33559.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A91506; DENCEN.
RefSeq XP_961561.1; -.
3D structure databases
HSSP P24295; 1AUP. [HSSP ENTRY / PDB]
ModBase P00369.
Enzyme and pathway databases
BioCyc MetaCyc:MON-13461; -.
Family and domain databases
InterPro IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11606:SF2; GLFV_DH; 1.
Pfam PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000185; Glu_DH; 1.
PRINTS PR00082; GLFDHDRGNASE.
PROSITE PS00074; GLFV_DEHYDROGENASE; 1.
BLOCKS P00369.
Genome annotation databases
GeneID 3877684; -.
KEGG ncr:NCU01195; -.
NMPDR fig|5141.1.peg.4621; -.
Other
ProtoNet P00369.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Complete proteome; Direct protein sequencing; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   454  453     NADP-specific glutamate dehydrogenase. PRO_0000182790
ACT_SITE   114   114        By similarity. 
MOD_RES   2     2        N-acetylserine. 
CONFLICT   57    57        N -> D (in Ref. 4; AA sequence). 
CONFLICT   314   315        QN -> ED (in Ref. 4; AA sequence). 
CONFLICT   451   451        Missing (in Ref. 4; AA sequence). 
Sequence information
Length: 454 AA [This is the length of the unprocessed precursor] Molecular weight: 48853 Da [This is the MW of the unprocessed precursor] CRC64: 908B56A63837DA28 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSNLPSEPEF EQAYKELAYT LENSSLFQKH PEYRTALTVA SIPERVIQFR VVWEDDNGNV 

        70         80         90        100        110        120 
QVNRGYRVQF NSALGPYKGG LRLHPSVNLS ILKFLGFEQI FKNALTGLSM GGGKGGADFD 

       130        140        150        160        170        180 
PKGKSDAEIR RFCCAFMAEL HKHIGADTDV PAGDIGVGGR EIGYMFGAYR KAANRFEGVL 

       190        200        210        220        230        240 
TGKGLSWGGS LIRPEATGYG LVYYVGHMLE YSGAGSYAGK RVALSGSGNV AQYAALKLIE 

       250        260        270        280        290        300 
LGATVVSLSD SKGALVATGE SGITVEDINA VMAIKEARQS LTSFQHAGHL KWIEGARPWL 

       310        320        330        340        350        360 
HVGKVDIALP CATQNEVSKE EAEGLLAAGC KFVAEGSNMG CTLEAIEVFE NNRKEKKGEA 

       370        380        390        400        410        420 
VWYAPGKAAN CGGVAVSGLE MAQNSQRLNW TQAEVDEKLK DIMKNAFFNG LNTAKTYVEA 

       430        440        450 
AEGELPSLVA GSNIAGFVKV AQAMHDQGDW WSKN 

P00369 in FASTA format

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