ID   DYR_BOVIN               Reviewed;         187 AA.
AC   P00376; Q29RI1;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-NOV-2008, entry version 55.
DE   RecName: Full=Dihydrofolate reductase;
DE            EC=1.5.1.3;
GN   Name=DHFR;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Heart ventricle;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 2-187.
RC   TISSUE=Liver;
RX   MEDLINE=83000246; PubMed=7115669; DOI=10.1021/bi00257a006;
RA   Lai P.-H., Pan Y.-C.E., Gleisner J.M., Peterson D.L., Williams K.R.,
RA   Blakley R.L.;
RT   "Structure of dihydrofolate reductase: primary sequence of the bovine
RT   liver enzyme.";
RL   Biochemistry 21:3284-3294(1982).
CC   -!- CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-
CC       dihydrofolate + NADPH.
CC   -!- PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis;
CC       tetrahydrofolate from dihydrofolate: step 1/1.
CC   -!- MISCELLANEOUS: The reaction catalyzed by this enzyme represents an
CC       essential step for de novo glycine and purine synthesis, DNA
CC       precursor synthesis, and for the conversion of dUMP to dTMP.
CC   -!- SIMILARITY: Belongs to the dihydrofolate reductase family.
CC   -!- SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; BC114162; AAI14163.1; -; mRNA.
DR   PIR; A00388; RDBOD.
DR   RefSeq; NP_001071351.1; -.
DR   UniGene; Bt.18730; -.
DR   HSSP; P00374; 1DHF.
DR   SMR; P00376; 2-187.
DR   Ensembl; ENSBTAG00000007681; Bos taurus.
DR   GeneID; 508809; -.
DR   KEGG; bta:508809; -.
DR   HOVERGEN; P00376; -.
DR   GO; GO:0004146; F:dihydrofolate reductase activity; IEA:InterPro.
DR   GO; GO:0006545; P:glycine biosynthetic process; IEA:InterPro.
DR   GO; GO:0009165; P:nucleotide biosynthetic process; IEA:InterPro.
DR   GO; GO:0006730; P:one-carbon compound metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001796; DHFR_reg.
DR   Pfam; PF00186; DHFR_1; 1.
DR   PRINTS; PR00070; DHFR.
DR   PROSITE; PS00075; DHFR_1; 1.
DR   PROSITE; PS51330; DHFR_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NADP; One-carbon metabolism;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    187       Dihydrofolate reductase.
FT                                /FTId=PRO_0000186361.
FT   DOMAIN        4    185       DHFR.
FT   CONFLICT     22     22       D -> N (in Ref. 1; AAI14163).
FT   CONFLICT    102    102       E -> Q (in Ref. 2; AA sequence).
SQ   SEQUENCE   187 AA;  21604 MW;  0727DD855C8A7CD8 CRC64;
     MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFQYFQRMTT VSSVEGKQNL VIMGRKTWFS
     IPEKNRPLKD RINIVLSREL KEPPKGAHFL AKSLDDALEL IEDPELTNKV DVVWIVGGSS
     VYKEAMNKPG HVRLFVTRIM QEFESDAFFP EIDFEKYKLL PEYPGVPLDV QEEKGIKYKF
     EVYEKNN
//