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UniProtKB/Swiss-Prot entry P01574


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IFNB_HUMAN
Primary accession number P01574
Secondary accession numbers None
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on July 21, 1986 (Sequence version 1)
Annotations were last modified on    June 10, 2008 (Entry version 101)
Name and origin of the protein
Protein name Interferon beta [Precursor]
Synonyms IFN-beta
Fibroblast interferon
Gene name
Name: IFNB1
Synonyms: IFB, IFNB
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE.
DOI=10.1093/nar/9.5.1045; PubMed=6164984 [NCBI, ExPASy, EBI, Israel, Japan]
Lawn R.M., Adelman J., Franke A.E., Houck C.M., Gross M., Najarian R., Goeddel D.V.;
"Human fibroblast interferon gene lacks introns.";
Nucleic Acids Res. 9:1045-1052(1981).
[2]
NUCLEOTIDE SEQUENCE.
PubMed=16593086 [NCBI, ExPASy, EBI, Israel, Japan]
Ohno S., Taniguchi T.;
"Structure of a chromosomal gene for human interferon beta.";
Proc. Natl. Acad. Sci. U.S.A. 78:5305-5309(1981).
[3]
NUCLEOTIDE SEQUENCE.
DOI=10.1016/0378-1119(80)90138-9; PubMed=6157601 [NCBI, ExPASy, EBI, Israel, Japan]
Taniguchi T., Ohno S., Fujii-Kuriyama Y., Muramatsu M.;
"The nucleotide sequence of human fibroblast interferon cDNA.";
Gene 10:11-15(1980).
[4]
NUCLEOTIDE SEQUENCE.
DOI=10.1038/285542a0; PubMed=6157094 [NCBI, ExPASy, EBI, Israel, Japan]
Derynck R., Content J., Declercq E., Volckaert G., Tavernier J., Devos R., Fiers W.;
"Isolation and structure of a human fibroblast interferon gene.";
Nature 285:542-547(1980).
[5]
NUCLEOTIDE SEQUENCE.
DOI=10.1093/nar/8.13.2885; PubMed=6159580 [NCBI, ExPASy, EBI, Israel, Japan]
Houghton M., Eaton M.A.W., Stewart A.G., Smith J.C., Doel S.M., Cartlin G.H., Lewis H.M., Patel T.P., Emtage J.S., Carey N.H., Porter A.G.;
"The complete amino acid sequence of human fibroblast interferon as deduced using synthetic oligodeoxyribonucleotide primers of reverse transcriptase.";
Nucleic Acids Res. 8:2885-2894(1980).
[6]
NUCLEOTIDE SEQUENCE.
DOI=10.1093/nar/8.18.4057; PubMed=6159584 [NCBI, ExPASy, EBI, Israel, Japan]
Goeddel D.V., Shepard H.M., Yelverton E., Leung D., Crea R., Sloma A., Pestka S.;
"Synthesis of human fibroblast interferon by E. coli.";
Nucleic Acids Res. 8:4057-4074(1980).
[7]
NUCLEOTIDE SEQUENCE.
PubMed=2414376 [NCBI, ExPASy, EBI, Israel, Japan]
May L.T., Sehgal P.B.;
"On the relationship between human interferon alpha 1 and beta 1 genes.";
J. Interferon Res. 5:521-526(1985).
[8]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[9]
NUCLEOTIDE SEQUENCE OF 1-68.
DOI=10.1093/nar/8.9.1913; PubMed=6159597 [NCBI, ExPASy, EBI, Israel, Japan]
Houghton M., Stewart A.G., Doel S.M., Emtage J.S., Eaton M.A.W., Smith J.C., Patel T.P., Lewis H.M., Porter A.G., Birch J.R., Cartwright T., Carey N.H.;
"The amino-terminal sequence of human fibroblast interferon as deduced from reverse transcripts obtained using synthetic oligonucleotide primers.";
Nucleic Acids Res. 8:1913-1931(1980).
[10]
DISULFIDE BOND.
DOI=10.1038/289606a0; PubMed=6162107 [NCBI, ExPASy, EBI, Israel, Japan]
Wetzel R.;
"Assignment of the disulphide bonds of leukocyte interferon.";
Nature 289:606-607(1981).
[11]
NUCLEOTIDE SEQUENCE OF 71-187 (VARIANT CLONE PF526).
DOI=10.1038/294563a0; PubMed=6171735 [NCBI, ExPASy, EBI, Israel, Japan]
Shepard H.M., Leung D., Stebbing N., Goeddel D.V.;
"A single amino acid change in IFN-beta1 abolishes its antiviral activity.";
Nature 294:563-565(1981).
[12]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-140, AND MASS SPECTROMETRY.
DOI=10.2116/analsci.24.161; PubMed=18187866 [NCBI, ExPASy, EBI, Israel, Japan]
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.;
"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column.";
Anal. Sci. 24:161-166(2008).
[13]
X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
DOI=10.1073/pnas.94.22.11813; PubMed=9342320 [NCBI, ExPASy, EBI, Israel, Japan]
Karpusas M., Nolte M., Benton C.B., Meier W., Lipscomb W.N., Goelz S.;
"The crystal structure of human interferon beta at 2.2-A resolution.";
Proc. Natl. Acad. Sci. U.S.A. 94:11813-11818(1997).
[14]
VARIANT [LARGE SCALE ANALYSIS] CYS-164.
DOI=10.1126/science.1133427; PubMed=16959974 [NCBI, ExPASy, EBI, Israel, Japan]
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.;
"The consensus coding sequences of human breast and colorectal cancers.";
Science 314:268-274(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
V00534; CAA23795.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00535; CAA23796.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00546; CAA23807.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00547; CAA23808.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC069314; AAH69314.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M28622; AAA36040.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A93721; IVHUB1.
RefSeq NP_002167.1; -.
UniGene Hs.93177
3D structure databases
PDB
1AU1; X-ray; 2.20 A; A/B=22-187.[ExPASy / RCSB / EBI]
PDBsum 1AU1; -.
ModBase P01574.
Protein-protein interaction databases
DIP DIP:6018N; -.
Organism-specific databases
H-InvDB HIX0034856; -.
HGNC HGNC:5434; IFNB1.
GeneLynx IFNB1; Homo sapiens.
GenAtlas IFNB1.
MIM 147640; gene. [NCBI / EBI]
PharmGKB PA29672; -.
GeneCards P01574.
Gene expression databases
ArrayExpress P01574; -.
CleanEx HS_IFNB1; -.
GermOnline ENSG00000171855; Homo sapiens.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred by curator from UniProtKB).
GO:0005132; Molecular function: interferon-alpha/beta receptor binding (non-traceable author statement from UniProtKB).
GO:0042100; Biological process: B cell proliferation (non-traceable author statement from UniProtKB).
GO:0006919; Biological process: caspase activation (inferred from direct assay from UniProtKB).
GO:0007166; Biological process: cell surface receptor linked signal transduction (traceable author statement from ProtInc).
GO:0006917; Biological process: induction of apoptosis (inferred from direct assay from UniProtKB).
GO:0030101; Biological process: natural killer cell activation (non-traceable author statement from UniProtKB).
GO:0008285; Biological process: negative regulation of cell proliferation (non-traceable author statement from UniProtKB).
GO:0046597; Biological process: negative regulation of virion penetration into host cell (non-traceable author statement from UniProtKB).
GO:0045089; Biological process: positive regulation of innate immune response (non-traceable author statement from UniProtKB).
GO:0045343; Biological process: regulation of MHC class I biosynthetic process (non-traceable author statement from UniProtKB).
GO:0009615; Biological process: response to virus (non-traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR012351; 4_helix_cytokine_core.
IPR015588; IFNb.
IPR000471; Interferon_abd.
Graphical view of domain structure.
Gene3D G3DSA:1.20.1250.10; 4_helix_cytokine_core; 1.
PANTHER PTHR11691:SF7; IFNb; 1.
PTHR11691; Interferon_abd; 1.
Pfam PF00143; Interferon; 1.
Pfam graphical view of domain structure.
PRINTS PR00266; INTERFERONAB.
ProDom PD000550; Interferon_abd; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00076; IFabd; 1.
SMART graphical view of domain structure.
PROSITE PS00252; INTERFERON_A_B_D; 1.
BLOCKS P01574.
Genome annotation databases
Ensembl ENSG00000171855; Homo sapiens. [Contig view]
GeneID 3456; -.
KEGG hsa:3456; -.
Phylogenomic databases
HOGENOM P01574; -.
HOVERGEN P01574; -.
Other
DrugBank DB00060; Interferon beta-1a.
DB00068; Interferon beta-1b.
LinkHub P01574; -.
SOURCE IFNB1; Homo sapiens.
ProtoNet P01574.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Antiviral defense; Cytokine; Glycoprotein; Pharmaceutical; Phosphoprotein; Polymorphism; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    21  21      
CHAIN   22   187  166     Interferon beta. PRO_0000016400
MOD_RES   140   140        Phosphoserine. 
CARBOHYD   101   101        N-linked (GlcNAc...). 
DISULFID   52   162         
VARIANT   162   162  1     C -> Y (in clone PF526, loss of ability to form the essential disulfide bond, loss of antiviral activity). VAR_004016 [3D]
VARIANT   164   164  1     W -> C (in a breast cancer sample; somatic mutation). VAR_036330 [3D]
HELIX   24    42  19      
HELIX   51    55  5      
HELIX   63    67  5      
HELIX   73    91  19      
HELIX   96    98  3      
HELIX   102   127  26      
TURN   137   139  3      
HELIX   140   156  17      
TURN   157   159  3      
HELIX   161   182  22      
Sequence information
Length: 187 AA [This is the length of the unprocessed precursor] Molecular weight: 22294 Da [This is the MW of the unprocessed precursor] CRC64: 0B013D4087723CEC [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTNKCLLQIA LLLCFSTTAL SMSYNLLGFL QRSSNFQCQK LLWQLNGRLE YCLKDRMNFD 

        70         80         90        100        110        120 
IPEEIKQLQQ FQKEDAALTI YEMLQNIFAI FRQDSSSTGW NETIVENLLA NVYHQINHLK 

       130        140        150        160        170        180 
TVLEEKLEKE DFTRGKLMSS LHLKRYYGRI LHYLKAKEYS HCAWTIVRVE ILRNFYFINR 


LTGYLRN 

P01574 in FASTA format

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