[1]	PROTEIN SEQUENCE. STRAIN=T47/F62; PubMed=6434541 [NCBI, ExPASy, EBI, Israel, Japan] Baccanari D.P., Tansik R.L., Paterson S.J., Stone D.; "Characterization and amino acid sequence of Neisseria gonorrhoeae dihydrofolate reductase."; J. Biol. Chem. 259:12291-12298(1984).

Comments

CATALYTIC ACTIVITY: 5,6,7,8-tetrahydrofolate + NADP⁺ = 7,8-dihydrofolate + NADPH.
PATHWAY: Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1 .
MISCELLANEOUS: Strain T47/F62 is trimethoprim resistant by overproduction of DHFR.
MISCELLANEOUS: The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
SIMILARITY: Belongs to the dihydrofolate reductase family.
SIMILARITY: Contains 1 DHFR (dihydrofolate reductase) domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases
PIR	A00393; RDNHD.
3D structure databases
HSSP	O33305; 1DF7. [HSSP ENTRY / PDB]
ModBase	P04174.
Family and domain databases
InterPro	IPR012259; DHFR. IPR001796; DHFR_reg. Graphical view of domain structure.
PANTHER	PTHR11549:SF1; DHFR; 1.
Pfam	PF00186; DHFR_1; 1. Pfam graphical view of domain structure.
PRINTS	PR00070; DHFR.
PROSITE	PS00075; DHFR_1; 1. PS51330; DHFR_2; 1. PROSITE graphical view of domain structure (profiles).
BLOCKS	P04174.
Other
ProtoNet	P04174.
UniRef	View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Antibiotic resistance; Direct protein sequencing; NADP; One-carbon metabolism; Oxidoreductase; Trimethoprim resistance.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 162`	`162`	`Dihydrofolate reductase.`	`PRO_0000186401`
`DOMAIN`	`3 161`	`159`	`DHFR.`

Sequence information

Length: 162 AA [This is the length of the unprocessed precursor]

Molecular weight: 17731 Da [This is the MW of the unprocessed precursor]

CRC64: A23BA94E6764E564 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLKITIIAAC AENLCIGAGN AMPWHIPEDF AFFKVYTLGK PVIMGRKTWE SLPVKPLPGR 

        70         80         90        100        110        120 
RNIVISRQAD YCAAGAETVA SLEVALALCA GAEEAVIMGG AQIYGQAMPL ATDLRITEVD 

       130        140        150        160 
LSVEGDAFFP EIDRTHWREA ERTERRVSSK GVAYTFVHYL GK

P04174 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools