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UniProtKB/Swiss-Prot entry P04382


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DYR_BPT4
Primary accession number P04382
Secondary accession numbers None
Integrated into Swiss-Prot on March 20, 1987
Sequence was last modified on March 20, 1987 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 61)
Name and origin of the protein
Protein name Dihydrofolate reductase
Synonym EC 1.5.1.3
Gene name
Name: frd
From
Bacteriophage T4 [TaxID: 10665] 
Taxonomy Viruses; dsDNA viruses, no RNA stage; Caudovirales; Myoviridae; T4-like viruses.
Virus host Escherichia coli [TaxID: 562]
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6327673 [NCBI, ExPASy, EBI, Israel, Japan]
Purohit S., Mathews C.K.;
"Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase.";
J. Biol. Chem. 259:6261-6266(1984).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/MMBR.67.1.86-156.2003; PubMed=12626685 [NCBI, ExPASy, EBI, Israel, Japan]
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
"Bacteriophage T4 genome.";
Microbiol. Mol. Biol. Rev. 67:86-156(2003).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 123-193.
STRAIN=ALC4;
DOI=10.1016/0092-8674(86)90379-X; PubMed=3698096 [NCBI, ExPASy, EBI, Israel, Japan]
Chu F.K., Maley G.F., West D.K., Belfort M., Maley F.;
"Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript.";
Cell 45:157-166(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
K01804; AAA32491.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF158101; AAD42577.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M12742; AAC12815.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A00396; RDBPT4.
RefSeq NP_049850.1; -.
3D structure databases
PDB
1JUV; X-ray; 1.70 A; A=1-193.[ExPASy / RCSB / EBI]
PDBsum 1JUV; -.
ModBase P04382.
Family and domain databases
InterPro IPR012259; DHFR.
IPR001796; DHFR_reg.
Graphical view of domain structure.
PANTHER PTHR11549:SF1; DHFR; 1.
Pfam PF00186; DHFR_1; 1.
Pfam graphical view of domain structure.
PRINTS PR00070; DHFR.
PROSITE PS00075; DHFR_1; 1.
PS51330; DHFR_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P04382.
Genome annotation databases
GeneID 1258671; -.
GenomeReviews AF158101_GR; T4p240.
Other
ProtoNet P04382.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Antibiotic resistance; Methotrexate resistance; NADP; One-carbon metabolism; Oxidoreductase; Trimethoprim resistance.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   193  193     Dihydrofolate reductase. PRO_0000186434
DOMAIN   1   193  193     DHFR. 
STRAND   2    12  11      
STRAND   18    24  7      
HELIX   34    44  11      
STRAND   47    52  6      
HELIX   53    57  5      
STRAND   68    71  4      
STRAND   88    91  4      
HELIX   93   100  8      
STRAND   105   108  4      
STRAND   115   118  4      
STRAND   124   128  5      
HELIX   130   136  7      
HELIX   137   139  3      
STRAND   141   152  12      
STRAND   157   160  4      
HELIX   162   169  8      
STRAND   173   182  10      
STRAND   185   192  8      
Sequence information
Length: 193 AA [This is the length of the unprocessed precursor] Molecular weight: 21713 Da [This is the MW of the unprocessed precursor] CRC64: 64E033916E628178 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MIKLVFRYSP TKTVDGFNEL AFGLGDGLPW GRVKKDLQNF KARTEGTIMI MGAKTFQSLP 

        70         80         90        100        110        120 
TLLPGRSHIV VCDLARDYPV TKDGDLAHFY ITWEQYITYI SGGEIQVSSP NAPFETMLDQ 

       130        140        150        160        170        180 
NSKVSVIGGP ALLYAALPYA DEVVVSRIVK RHRVNSTVQL DASFLDDISK REMVETHWYK 

       190 
IDEVTTLTES VYK 

P04382 in FASTA format

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