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UniProtKB/Swiss-Prot entry P04904


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GSTA3_RAT
Primary accession number P04904
Secondary accession number Q6LD92
Integrated into Swiss-Prot on August 13, 1987
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    November 25, 2008 (Entry version 81)
Name and origin of the protein
Protein name Glutathione S-transferase alpha-3
Synonyms EC 2.5.1.18
Glutathione S-transferase Yc-1
GST Yc1
GST 2-2
GST AA
GST A3-3
Gene name
Name: Gsta3
Synonyms: Gstyc1
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Fischer 344;
TISSUE=Liver;
PubMed=8051171 [NCBI, ExPASy, EBI, Israel, Japan]
Hayes J.D., Nguyen T., Judah D.J., Petersson D.G., Neal G.E.;
"Cloning of cDNAs from fetal rat liver encoding glutathione S-transferase Yc polypeptides. The Yc2 subunit is expressed in adult rat liver resistant to the hepatocarcinogen aflatoxin B1.";
J. Biol. Chem. 269:20707-20717(1994).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2985614 [NCBI, ExPASy, EBI, Israel, Japan]
Telakowski-Hopkins C.A., Rodkey K.A., Bennett C.D., Lu A.Y.H., Pickett C.B.;
"Rat liver glutathione S-transferases. Construction of a cDNA clone complementary to a Yc mRNA and prediction of the complete amino acid sequence of a Yc subunit.";
J. Biol. Chem. 260:5820-5825(1985).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=Fischer;
TISSUE=Liver;
DOI=10.1042/0264-6021:3390685; PubMed=10215608 [NCBI, ExPASy, EBI, Israel, Japan]
Fotouhi-Ardakani N., Batist G.;
"Genomic cloning and characterization of the rat glutathione S-transferase-A3-subunit gene.";
Biochem. J. 339:685-693(1999).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Liver, and Pituitary;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PROTEIN SEQUENCE OF 2-13; 21-36; 70-78; 142-152; 156-182; 188-195 AND 197-204, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Brain, Hippocampus, and Spinal cord;
Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.;
Submitted (JUL-2007) to UniProtKB.
[6]
NUCLEOTIDE SEQUENCE [MRNA] OF 75-221.
PubMed=6204982 [NCBI, ExPASy, EBI, Israel, Japan]
Tu C.-P.D., Lai H.-C.J., Li N.-Q., Weiss M.J., Reddy C.C.;
"The Yc and Ya subunits of rat liver glutathione S-transferases are the products of separate genes.";
J. Biol. Chem. 259:9434-9439(1984).
[7]
PARTIAL PROTEIN SEQUENCE.
STRAIN=Fischer 344;
TISSUE=Liver;
PubMed=1953636 [NCBI, ExPASy, EBI, Israel, Japan]
Hayes J.D., Judah D.J., McLellan L.I., Kerr L.A., Peacock S.D., Neal G.E.;
"Ethoxyquin-induced resistance to aflatoxin B1 in the rat is associated with the expression of a novel alpha-class glutathione S-transferase subunit, Yc2, which possesses high catalytic activity for aflatoxin B1-8,9-epoxide.";
Biochem. J. 279:385-398(1991).
[8]
PROTEIN SEQUENCE OF 2-31.
DOI=10.1016/0003-9861(90)90470-J; PubMed=2186703 [NCBI, ExPASy, EBI, Israel, Japan]
Huskey S.E., Wang R.W., Linemeyer D.L., Pickett C.B., Lu A.Y.H.;
"Expression in Escherichia coli of rat liver cytosolic glutathione S-transferase Yc cDNA.";
Arch. Biochem. Biophys. 279:116-121(1990).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X78848; CAA55405.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S72505; AAP21064.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K01932; AAA41294.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF111160; AAD28714.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC059128; AAH59128.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC088127; AAH88127.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A26753; A26753.
RefSeq NP_113697.1; -.
UniGene Rn.10460
3D structure databases
HSSP P08263; 1K3O. [HSSP ENTRY / PDB]
SMR P04904; 4-220.
ModBase P04904.
Organism-specific databases
RGD 2753; Gsta3.
Gene expression databases
ArrayExpress P04904; -.
GermOnline ENSRNOG00000013484; Rattus norvegicus.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0004364; Molecular function: glutathione transferase activity (inferred from electronic annotation from InterPro).
GO:0008152; Biological process: metabolic process (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR010987; Glutathione-S-Trfase_C-like.
IPR003080; GST_alpha.
IPR004046; GST_C.
IPR004045; GST_N.
IPR012335; Thioredoxin_fold.
Graphical view of domain structure.
Gene3D G3DSA:1.20.1050.10; GST_C_like; 1.
G3DSA:3.40.30.10; Thioredoxin_fold; 1.
PANTHER PTHR11571:SF4; GST_alpha; 1.
Pfam PF00043; GST_C; 1.
PF02798; GST_N; 1.
Pfam graphical view of domain structure.
PRINTS PR01266; GSTRNSFRASEA.
PROSITE PS50405; GST_CTER; 1.
PS50404; GST_NTER; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P04904.
Genome annotation databases
Ensembl ENSRNOG00000013484; Rattus norvegicus. [Contig view]
GeneID 24421; -.
KEGG rno:24421; -.
NMPDR fig|10116.3.peg.29788; -.
Phylogenomic databases
HOVERGEN P04904; -.
Other
NextBio 603279; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cytoplasm; Direct protein sequencing; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   221  220     Glutathione S-transferase alpha-3. PRO_0000185794
DOMAIN   3    83  81     GST N-terminal. 
DOMAIN   85   207  123     GST C-terminal. 
CONFLICT   102   102        L -> I (in Ref. 2). 
CONFLICT   184   184        L -> K (in Ref. 2). 
Sequence information
Length: 221 AA [This is the length of the unprocessed precursor] Molecular weight: 25319 Da [This is the MW of the unprocessed precursor] CRC64: C45C5AF03573707F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPGKPVLHYF DGRGRMEPIR WLLAAAGVEF EEQFLKTRDD LARLRNDGSL MFQQVPMVEI 

        70         80         90        100        110        120 
DGMKLVQTRA ILNYIATKYN LYGKDMKERA LIDMYAEGVA DLDEIVLHYP YIPPGEKEAS 

       130        140        150        160        170        180 
LAKIKDKARN RYFPAFEKVL KSHGQDYLVG NRLSRADVYL VQVLYHVEEL DPSALANFPL 

       190        200        210        220 
LKALRTRVSN LPTVKKFLQP GSQRKPLEDE KCVESAVKIF S 

P04904 in FASTA format

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