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UniProtKB/Swiss-Prot entry P05314

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NIR_SPIOL
Primary accession number P05314
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1988
Sequence was last modified on November 1, 1988 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 79)
Name and origin of the protein
Protein name Ferredoxin--nitrite reductase, chloroplastic [Precursor]
Synonym EC 1.7.7.1
Gene name
Name: NIR
From
Spinacia oleracea (Spinach) [TaxID: 3562] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; Caryophyllales; Amaranthaceae; Spinacia.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
DOI=10.1007/BF00322440; PubMed=3163766 [NCBI, ExPASy, EBI, Israel, Japan]
Back E., Burkhart W., Moyer M., Privalle L., Rothstein S.;
"Isolation of cDNA clones coding for spinach nitrite reductase: complete sequence and nitrate induction.";
Mol. Gen. Genet. 212:20-26(1988).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=cv. Dark green Bloomsdale;
TISSUE=Leaf;
DOI=10.1007/BF00036801; PubMed=1868226 [NCBI, ExPASy, EBI, Israel, Japan]
Back E., Dunne W., Schneiderbauer A., de Framond A., Rastogi R., Rothstein S.J.;
"Isolation of the spinach nitrite reductase gene promoter which confers nitrate inducibility on GUS gene expression in transgenic tobacco.";
Plant Mol. Biol. 17:9-18(1991).
[3]
 X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), IRON-CLUSTER BINDING AT CYS-473; CYS-479; CYS-514 AND CYS-518, AND HEME BINDING AT CYS-518.
DOI=10.1021/bi050981y; PubMed=16331965 [NCBI, ExPASy, EBI, Israel, Japan]
Swamy U., Wang M., Tripathy J.N., Kim S.K., Hirasawa M., Knaff D.B., Allen J.P.;
"Structure of spinach nitrite reductase: implications for multi-electron reactions by the iron-sulfur:siroheme cofactor.";
Biochemistry 44:16054-16063(2005).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X07568; CAA30453.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X17031; CAA34893.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S16603; S16603.
3D structure databases
PDB
2AKJ; X-ray; 2.80 A; A=1-594.[ExPASy / RCSB / EBI]
PDBsum 2AKJ; -.
ModBase P05314.
Family and domain databases
InterPro IPR006066; Nir_Si_BS.
IPR006067; Nir_Sir_4Fe4S.
IPR005117; NiRdtase/SiRdtase_haem-b_fer.
Graphical view of domain structure.
Pfam PF01077; NIR_SIR; 2.
PF03460; NIR_SIR_ferr; 2.
Pfam graphical view of domain structure.
PRINTS PR00397; SIROHAEM.
PROSITE PS00365; NIR_SIR; 1.
BLOCKS P05314.
Other
ProtoNet P05314.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; 4Fe-4S; Chloroplast; Direct protein sequencing; Electron transport; Heme; Iron; Iron-sulfur; Metal-binding; Nitrate assimilation; Oxidoreductase; Plastid; Transit peptide; Transport.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    32  32     Chloroplast. 
CHAIN   33   594  562     Ferredoxin--nitrite reductase, chloroplastic. PRO_0000019706
METAL   473   473        Iron-sulfur (4Fe-4S). 
METAL   479   479        Iron-sulfur (4Fe-4S). 
METAL   514   514        Iron-sulfur (4Fe-4S). 
METAL   518   518        Iron (siroheme axial ligand). 
METAL   518   518        Iron-sulfur (4Fe-4S). 
VARIANT   221   221  1     I -> V (probable allelic variation). 
STRAND   58    62  5      
STRAND   65    68  4      
HELIX   70    72  3      
HELIX   78    85  8      
HELIX   90    93  4      
HELIX   96   100  5      
HELIX   105   108  4      
HELIX   112   119  8      
HELIX   121   125  5      
STRAND   127   129  3      
HELIX   131   134  4      
STRAND   138   141  4      
HELIX   145   147  3      
STRAND   148   150  3      
HELIX   151   162  12      
HELIX   163   167  5      
STRAND   170   172  3      
STRAND   178   183  6      
HELIX   185   187  3      
HELIX   188   196  9      
TURN   197   199  3      
STRAND   206   210  5      
TURN   217   221  5      
HELIX   230   240  11      
TURN   241   245  5      
HELIX   248   250  3      
STRAND   259   261  3      
HELIX   271   273  3      
STRAND   274   293  20      
STRAND   306   313  8      
HELIX   314   316  3      
HELIX   317   331  15      
HELIX   337   339  3      
HELIX   342   349  8      
HELIX   351   359  9      
STRAND   386   390  5      
STRAND   396   401  6      
HELIX   404   406  3      
HELIX   410   423  14      
STRAND   424   431  8      
TURN   432   434  3      
STRAND   435   442  8      
TURN   443   445  3      
HELIX   446   449  4      
HELIX   453   456  4      
TURN   465   468  4      
STRAND   469   472  4      
HELIX   475   477  3      
HELIX   487   500  14      
STRAND   508   514  7      
HELIX   521   523  3      
STRAND   524   534  11      
STRAND   540   548  9      
STRAND   560   567  8      
TURN   568   570  3      
HELIX   571   582  12      
Sequence information
Length: 594 AA [This is the length of the unprocessed precursor] Molecular weight: 66394 Da [This is the MW of the unprocessed precursor] CRC64: 2B3DCAAC16DE06A3 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MASLPVNKII PSSTTLLSSS NNNRRRNNSS IRCQKAVSPA AETAAVSPSV DAARLEPRVE 

        70         80         90        100        110        120 
ERDGFWVLKE EFRSGINPAE KVKIEKDPMK LFIEDGISDL ATLSMEEVDK SKHNKDDIDV 

       130        140        150        160        170        180 
RLKWLGLFHR RKHHYGRFMM RLKLPNGVTT SEQTRYLASV IKKYGKDGCA DVTTRQNWQI 

       190        200        210        220        230        240 
RGVVLPDVPE IIKGLESVGL TSLQSGMDNV RNPVGNPLAG IDPHEIVDTR PFTNLISQFV 

       250        260        270        280        290        300 
TANSRGNLSI TNLPRKWNPC VIGSHDLYEH PHINDLAYMP ATKNGKFGFN LLVGGFFSIK 

       310        320        330        340        350        360 
RCEEAIPLDA WVSAEDVVPV CKAMLEAFRD LGFRGNRQKC RMMWLIDELG MEAFRGEVEK 

       370        380        390        400        410        420 
RMPEQVLERA SSEELVQKDW ERREYLGVHP QKQQGLSFVG LHIPVGRLQA DEMEELARIA 

       430        440        450        460        470        480 
DVYGSGELRL TVEQNIIIPN VENSKIDSLL NEPLLKERYS PEPPILMKGL VACTGSQFCG 

       490        500        510        520        530        540 
QAIIETKARA LKVTEEVQRL VSVTRPVRMH WTGCPNSCGQ VQVADIGFMG CMTRDENGKP 

       550        560        570        580        590 
CEGADVFVGG RIGSDSHLGD IYKKAVPCKD LVPVVAEILI NQFGAVPRER EEAE
P05314 in FASTA format

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