ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry P07262


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name DHE4_YEAST
Primary accession number P07262
Secondary accession number Q08899
Integrated into Swiss-Prot on April 1, 1988
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 86)
Name and origin of the protein
Protein name NADP-specific glutamate dehydrogenase 1
Synonyms NADP-GDH 1
EC 1.4.1.4
NADP-dependent glutamate dehydrogenase 1
Gene name
Name: GDH1
Synonyms: URE1
OrderedLocusNames: YOR375C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2989290 [NCBI, ExPASy, EBI, Israel, Japan]
Moye W.S., Amuro N., Rao J.K.M., Zalkin H.;
"Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase.";
J. Biol. Chem. 260:8502-8508(1985).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(85)90279-3; PubMed=2932370 [NCBI, ExPASy, EBI, Israel, Japan]
Nagasu T., Hall B.D.;
"Nucleotide sequence of the GDH gene coding for the NADP-specific glutamate dehydrogenase of Saccharomyces cerevisiae.";
Gene 37:247-253(1985).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169874 [NCBI, ExPASy, EBI, Israel, Japan]
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
Nature 387:98-102(1997).
[4]
PROTEIN SEQUENCE OF 79-88; 157-165 AND 236-246.
STRAIN=ATCC 38531 / Y41;
DOI=10.1016/0378-1097(96)00006-7; PubMed=8935650 [NCBI, ExPASy, EBI, Israel, Japan]
Norbeck J., Blomberg A.;
"Protein expression during exponential growth in 0.7 M NaCl medium of Saccharomyces cerevisiae.";
FEMS Microbiol. Lett. 137:1-8(1996).
[5]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[6]
UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-325 AND LYS-371, AND MASS SPECTROMETRY.
DOI=10.1038/nbt849; PubMed=12872131 [NCBI, ExPASy, EBI, Israel, Japan]
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.;
"A proteomics approach to understanding protein ubiquitination.";
Nat. Biotechnol. 21:921-926(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M11297; AAB03898.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M10590; AAA34642.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z75283; CAA99706.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S67287; A25275.
RefSeq NP_015020.1; -.
3D structure databases
HSSP P24295; 1AUP. [HSSP ENTRY / PDB]
ModBase P07262.
Protein-protein interaction databases
DIP DIP:1329N; -.
IntAct P07262; -.
Enzyme and pathway databases
BioCyc MetaCyc:MON-11504; -.
Organism-specific databases
CYGD YOR375c; -.
SGD S000005902; GDH1.
Yeast-GFP YOR375C.
Gene expression databases
ArrayExpress P07262; -.
GermOnline YOR375C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from direct assay from SGD).
GO:0005634; Cellular component: nucleus (inferred from direct assay from SGD).
GO:0004354; Molecular function: glutamate dehydrogenase (NADP+) activity (inferred from direct assay from SGD).
GO:0006537; Biological process: glutamate biosynthetic process (inferred from genetic interaction from SGD).
QuickGo view.
Family and domain databases
InterPro IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11606:SF2; GLFV_DH; 1.
Pfam PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000185; Glu_DH; 1.
PRINTS PR00082; GLFDHDRGNASE.
PROSITE PS00074; GLFV_DEHYDROGENASE; 1.
BLOCKS P07262.
Proteomic databases
PeptideAtlas P07262; -.
Genome annotation databases
Ensembl YOR375C; Saccharomyces cerevisiae. [Contig view]
GeneID 854557; -.
GenomeReviews Y13140_GR; YOR375C.
KEGG sce:YOR375C; -.
NMPDR fig|4932.3.peg.6139; -.
Phylogenomic databases
HOGENOM P07262; -.
Other
LinkHub P07262; -.
ProtoNet P07262.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; NADP; Oxidoreductase; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   454  454     NADP-specific glutamate dehydrogenase 1. PRO_0000182798
NP_BIND   174   203  30     NAD (By similarity). 
ACT_SITE   110   110        By similarity. 
CROSSLNK   325   325        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin). 
CROSSLNK   371   371        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin). 
CONFLICT   83    83        V -> G (in Ref. 1; AAB03898). 
CONFLICT   198   198        V -> L (in Ref. 1; AAB03898). 
CONFLICT   255   255        S -> L (in Ref. 1; AAB03898). 
CONFLICT   266   266        D -> V (in Ref. 1; AAB03898). 
CONFLICT   358   364        ATGPSEA -> PLDQAT (in Ref. 2; AAA34642). 
Sequence information
Length: 454 AA [This is the length of the unprocessed precursor] Molecular weight: 49570 Da [This is the MW of the unprocessed precursor] CRC64: 707F921B6FE5B5B1 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSEPEFQQAY EEVVSSLEDS TLFEQHPEYR KVLPIVSVPE RIIQFRVTWE NDKGEQEVAQ 

        70         80         90        100        110        120 
GYRVQYNSAK GPYKGGLRFH PSVNLSILKF LGFEQIFKNS LTGLDMGGGK GGLCVDLKGR 

       130        140        150        160        170        180 
SNNEIRRICY AFMRELSRHI GQDTDVPAGD IGVGGREIGY LFGAYRSYKN SWEGVLTGKG 

       190        200        210        220        230        240 
LNWGGSLIRP EATGYGLVYY TQAMIDYATN GKESFEGKRV TISGSGNVAQ YAALKVIELG 

       250        260        270        280        290        300 
GTVVSLSDSK GCIISETGIT SEQVADISSA KVNFKSLEQI VNEYSTFSEN KVQYIAGARP 

       310        320        330        340        350        360 
WTHVQKVDIA LPCATQNEVS GEEAKALVAQ GVKFIAEGSN MGSTPEAIAV FETARSTATG 

       370        380        390        400        410        420 
PSEAVWYGPP KAANLGGVAV SGLEMAQNSQ RITWTSERVD QELKRIMINC FNECIDYAKK 

       430        440        450 
YTKDGKVLPS LVKGANIASF IKVSDAMFDQ GDVF 

P07262 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ca flag CBR Canada Mirror sites: Australia  Brazil  China  Korea  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!