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UniProtKB/Swiss-Prot entry P09368


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PUT1_YEAST
Primary accession number P09368
Secondary accession numbers None
Integrated into Swiss-Prot on July 1, 1989
Sequence was last modified on February 1, 1996 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 72)
Name and origin of the protein
Protein name Proline oxidase, mitochondrial [Precursor]
Synonyms EC 1.5.99.8
Proline dehydrogenase
Gene name
Name: PUT1
OrderedLocusNames: YLR142W
ORFNames: L3170, L9606.2
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE.
PubMed=3125423 [NCBI, ExPASy, EBI, Israel, Japan]
Wang S.-S., Brandriss M.C.;
"Proline utilization in Saccharomyces cerevisiae: sequence, regulation, and mitochondrial localization of the PUT1 gene product.";
Mol. Cell. Biol. 7:4431-4440(1987).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169871 [NCBI, ExPASy, EBI, Israel, Japan]
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
Nature 387:87-90(1997).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M18107; AAA16631.1; -; Unassigned_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X91258; CAA62662.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z73314; CAA97714.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U53881; AAB82390.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY692906; AAT92925.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S59339; S59339.
RefSeq NP_013243.1; -.
3D structure databases
ModBase P09368.
Protein-protein interaction databases
IntAct P09368; -.
Enzyme and pathway databases
BioCyc MetaCyc:MON-645; -.
Organism-specific databases
CYGD YLR142w; -.
SGD S000004132; PUT1.
Yeast-GFP YLR142W.
Gene expression databases
GermOnline YLR142W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from SGD).
GO:0004657; Molecular function: proline dehydrogenase activity (inferred from mutant phenotype from SGD).
GO:0006537; Biological process: glutamate biosynthetic process (traceable author statement from SGD).
GO:0010133; Biological process: proline catabolic process to glutamate (inferred from mutant phenotype from SGD).
QuickGo view.
Family and domain databases
InterPro IPR002872; Proline_DHase.
IPR015659; Proline_oxidase.
Graphical view of domain structure.
PANTHER PTHR13914; Proline_oxidase; 1.
Pfam PF01619; Pro_dh; 1.
Pfam graphical view of domain structure.
BLOCKS P09368.
Genome annotation databases
Ensembl YLR142W; Saccharomyces cerevisiae. [Contig view]
GeneID 850833; -.
GenomeReviews Y13138_GR; YLR142W.
KEGG sce:YLR142W; -.
NMPDR fig|4932.3.peg.4236; -.
Phylogenomic databases
HOGENOM P09368; -.
Other
LinkHub P09368; -.
ProtoNet P09368.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; FAD; Flavoprotein; Mitochondrion; Oxidoreductase; Proline metabolism; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1     ?        Mitochondrion (Potential). 
CHAIN   ?   476        Proline oxidase, mitochondrial. PRO_0000025806
CONFLICT   50    50        A -> P (in Ref. 1; AAA16631). 
CONFLICT   185   185        N -> T (in Ref. 1; AAA16631). 
Sequence information
Length: 476 AA [This is the length of the unprocessed precursor] Molecular weight: 53271 Da [This is the MW of the unprocessed precursor] CRC64: 12FEE55F86E1707D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MIASKSSLLV TKSRIPSLCF PLIKRSYVSK TPTHSNTAAN LMVETPAANA NGNSVMAPPN 

        70         80         90        100        110        120 
SINFLQTLPK KELFQLGFIG IATLNSFFLN TIIKLFPYIP IPVIKFFVSS LYCGGENFKE 

       130        140        150        160        170        180 
VIECGKRLQK RGISNMMLSL TIENSEGTKS LSSTPVDQIV KETISSVHNI LLPNIIGQLE 

       190        200        210        220        230        240 
SKPINDIAPG YIALKPSALV DNPHEVLYNF SNPAYKAQRD QLIENCSKIT KEIFELNQSL 

       250        260        270        280        290        300 
LKKYPERKAP FMVSTIDAEK YDLQENGVYE LQRILFQKFN PTSSKLISCV GTWQLYLRDS 

       310        320        330        340        350        360 
GDHILHELKL AQENGYKLGL KLVRGAYIHS EKNRNQIIFG DKTGTDENYD RIITQVVNDL 

       370        380        390        400        410        420 
IINGEDSYFG HLVVASHNYQ SQMLVTNLLK STQDNSYAKS NIVLGQLLGM ADNVTYDLIT 

       430        440        450        460        470 
NHGAKNIIKY VPWGPPLETK DYLLRRLQEN GDAVRSDNGW PLIKAIAKSI PKRVGL 

P09368 in FASTA format

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View entry in raw text format (no links)
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