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UniProtKB/Swiss-Prot entry P0A8N7

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SYK3_ECOLI
Primary accession number P0A8N7
Secondary accession numbers P03812 P78141 Q2M6E7 Q8XDP9
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on June 7, 2005 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 34)
Name and origin of the protein
Protein name Putative lysyl-tRNA synthetase
Synonyms EC 6.1.1.6
Lysine--tRNA ligase
LysRS
GX
Gene name
Name: poxA
Synonyms: genX, yjeA
OrderedLocusNames: b4155, JW4116
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(91)90503-4; PubMed=1761227 [NCBI, ExPASy, EBI, Israel, Japan]
Kong L., Fromant M., Blanquet S., Plateau P.;
"Evidence for a new Escherichia coli protein resembling a lysyl-tRNA synthetase.";
Gene 108:163-164(1991).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1093/nar/23.12.2105; PubMed=7610040 [NCBI, ExPASy, EBI, Israel, Japan]
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.";
Nucleic Acids Res. 23:2105-2119(1995).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-152.
PubMed=7037404 [NCBI, ExPASy, EBI, Israel, Japan]
Cole S.T.;
"Nucleotide sequence coding for the flavoprotein subunit of the fumarate reductase of Escherichia coli.";
Eur. J. Biochem. 122:479-484(1982).
[6]
 SIMILARITY TO CLASS-II AA-TRNA SYNTHETASES.
PubMed=2668951 [NCBI, ExPASy, EBI, Israel, Japan]
Gampel A., Tzagoloff A.;
"Homology of aspartyl- and lysyl-tRNA synthetases.";
Proc. Natl. Acad. Sci. U.S.A. 86:6023-6027(1989).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X59988; CAA42604.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U14003; AAA97054.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC77115.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE78159.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
J01611; AAA23436.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S56383; S56383.
RefSeq AP_004658.1; -.
NP_418579.2; -.
3D structure databases
HSSP P14825; 1E1O. [HSSP ENTRY / PDB]
ModBase P0A8N7.
Protein-protein interaction databases
IntAct P0A8N7; -.
Enzyme and pathway databases
BioCyc EcoCyc:EG11211-MON; -.
Organism-specific databases
EchoBASE EB1196; -.
EcoGene EG11211; poxA.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from InterPro).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from HAMAP).
GO:0004824; Molecular function: lysine-tRNA ligase activity (inferred from electronic annotation from HAMAP).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006430; Biological process: lysyl-tRNA aminoacylation (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00174; -; 1.
PBIL [Tree]
InterPro IPR004364; aa-tRNA-synt_II.
IPR006195; aa-tRNA-synth_II.
IPR004525; Lys-tRNA-synth-rel.
IPR002313; Lys-tRNA-synth_II.
Graphical view of domain structure.
PANTHER PTHR22594; aa-tRNA-synt_II; 1.
Pfam PF00152; tRNA-synt_2; 1.
Pfam graphical view of domain structure.
PRINTS PR00982; TRNASYNTHLYS.
TIGRFAMs TIGR00462; genX; 1.
PROSITE PS50862; AA_TRNA_LIGASE_II; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P0A8N7.
Genome annotation databases
GeneID 948672; -.
GenomeReviews U00096_GR; b4155.
AP009048_GR; JW4116.
KEGG ecj:JW4116; -.
eco:b4155; -.
Phylogenomic databases
HOGENOM P0A8N7; -.
Genome annotation databases
CMR P0A8N7; b4155.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Ligase; Nucleotide-binding; Protein biosynthesis.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   325  325     Putative lysyl-tRNA synthetase. PRO_0000152719
CONFLICT   78    78        E -> Q (in Ref. 5; AAA23436). 
CONFLICT   144   144        A -> E (in Ref. 5; AAA23436). 
Sequence information
Length: 325 AA [This is the length of the unprocessed precursor] Molecular weight: 36976 Da [This is the MW of the unprocessed precursor] CRC64: F72B535CCA23E4CC [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSETASWQPS ASIPNLLKRA AIMAEIRRFF ADRGVLEVET PCMSQATVTD IHLVPFETRF 

        70         80         90        100        110        120 
VGPGHSQGMN LWLMTSPEYH MKRLLVAGCG PVFQLCRSFR NEEMGRYHNP EFTMLEWYRP 

       130        140        150        160        170        180 
HYDMYRLMNE VDDLLQQVLD CPAAESLSYQ QAFLRYLEID PLSADKTQLR EVAAKLDLSN 

       190        200        210        220        230        240 
VADTEEDRDT LLQLLFTFGV EPNIGKEKPT FVYHFPASQA SLAQISTEDH RVAERFEVYY 

       250        260        270        280        290        300 
KGIELANGFH ELTDAREQQQ RFEQDNRKRA ARGLPQHPID QNLIEALKVG MPDCSGVALG 

       310        320 
VDRLVMLALG AETLAEVIAF SVDRA
P0A8N7 in FASTA format

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