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UniProtKB/Swiss-Prot entry P0AEA8


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CYSG_ECOLI
Primary accession number P0AEA8
Secondary accession numbers P11098 P76685 Q2M734
Integrated into Swiss-Prot on December 6, 2005
Sequence was last modified on December 6, 2005 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 34)
Name and origin of the protein
Protein name Siroheme synthase
Synonyms None
Includes Uroporphyrinogen-III C-methyltransferase
     (Urogen III methylase)
     (EC 2.1.1.107)
     (SUMT)
     (Uroporphyrinogen III methylase)
     (UROM)
Precorrin-2 dehydrogenase
     (EC 1.3.1.76)
Sirohydrochlorin ferrochelatase
     (EC 4.99.1.4)
Gene name
Name: cysG
OrderedLocusNames: b3368, JW3331
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
DOI=10.1093/nar/17.10.3865; PubMed=2543955 [NCBI, ExPASy, EBI, Israel, Japan]
Bell A.I., Gaston K.L., Cole J.A., Busby S.J.W.;
"Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP.";
Nucleic Acids Res. 17:3865-3874(1989).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=K12;
PubMed=2200672 [NCBI, ExPASy, EBI, Israel, Japan]
Peakman T., Crouzet J., Mayaux J.F., Busby S.J.W., Mohan S., Harborne N., Wootton J., Nicolson R., Cole J.A.;
"Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB-cysG region of the Escherichia coli K-12 chromosome.";
Eur. J. Biochem. 191:315-323(1990).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[5]
FUNCTION, AND PROTEIN SEQUENCE OF 1-18.
DOI=10.1016/0014-5793(90)80640-5; PubMed=2407558 [NCBI, ExPASy, EBI, Israel, Japan]
Warren M.J., Stolowich N.J., Santander P.J., Roessner C.A., Sowa B.A., Scott A.I.;
"Enzymatic synthesis of dihydrosirohydrochlorin (precorrin-2) and of a novel pyrrocorphin by uroporphyrinogen III methylase.";
FEBS Lett. 261:76-80(1990).
[6]
FUNCTION.
PubMed=2407234 [NCBI, ExPASy, EBI, Israel, Japan]
Warren M.J., Roessner C.A., Santander P.J., Scott A.I.;
"The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase.";
Biochem. J. 265:725-729(1990).
[7]
FUNCTION.
DOI=10.1016/0014-5793(93)80438-Z; PubMed=8243665 [NCBI, ExPASy, EBI, Israel, Japan]
Spencer J.B., Stolowich N.J., Roessner C.A., Scott A.I.;
"The Escherichia coli cysG gene encodes the multifunctional protein, siroheme synthase.";
FEBS Lett. 335:57-60(1993).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X14202; CAA32419.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U18997; AAA58165.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC76393.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE77922.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C65131; C65131.
RefSeq AP_004421.1; -.
NP_417827.1; -.
3D structure databases
HSSP O87696; 1CBF. [HSSP ENTRY / PDB]
SMR P0AEA8; 1-457.
ModBase P0AEA8.
Protein-protein interaction databases
IntAct P0AEA8; -.
Enzyme and pathway databases
BioCyc EcoCyc:SIROHEMESYN-MON; -.
MetaCyc:SIROHEMESYN-MON; -.
Organism-specific databases
EchoBASE EB0185; -.
EcoGene EG10188; cysG.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
HAMAP MF_01646; -; 1.
PBIL [Tree]
InterPro IPR000878; 4pyrrol_Mease.
IPR014777; 4pyrrole_Mease_sub1.
IPR014776; 4pyrrole_Mease_sub2.
IPR006366; CobA_cysG_C.
IPR006367; CysG_synth_N.
IPR016040; NAD(P)-bd.
IPR003043; Uropor_MeTrfase_CS.
Graphical view of domain structure.
Gene3D G3DSA:3.40.1010.10; 4pyrrole_Mease_sub1; 1.
G3DSA:3.30.950.10; 4pyrrole_Mease_sub2; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00590; TP_methylase; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01469; cobA_cysG_Cterm; 1.
TIGR01470; cysG_Nterm; 1.
PROSITE PS00839; SUMT_1; 1.
PS00840; SUMT_2; 1.
BLOCKS P0AEA8.
Genome annotation databases
GeneID 947880; -.
GenomeReviews U00096_GR; b3368.
AP009048_GR; JW3331.
KEGG ecj:JW3331; -.
eco:b3368; -.
Phylogenomic databases
HOGENOM P0AEA8; -.
Genome annotation databases
CMR P0AEA8; b3368.
Other
ProtoNet P0AEA8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cobalamin biosynthesis; Complete proteome; Direct protein sequencing; Lyase; Methyltransferase; Multifunctional enzyme; NAD; Oxidoreductase; Porphyrin biosynthesis; S-adenosyl-L-methionine; Transferase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   457  457     Siroheme synthase. PRO_0000150378
REGION   218   457  240     Uroporphyrinogen-III C-methyltransferase. 
CONFLICT   26    26        R -> P (in Ref. 1 and 2). 
CONFLICT   90    91        SE -> RQ (in Ref. 1 and 2). 
Sequence information
Length: 457 AA [This is the length of the unprocessed precursor] Molecular weight: 49951 Da [This is the MW of the unprocessed precursor] CRC64: DDDE0DC90C5CF2F4 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLDAGARLTV NALAFIPQFT AWADAGMLTL 

        70         80         90        100        110        120 
VEGPFDESLL DTCWLAIAAT DDDALNQRVS EAAEARRIFC NVVDAPKAAS FIMPSIIDRS 

       130        140        150        160        170        180 
PLMVAVSSGG TSPVLARLLR EKLESLLPLH LGQVAKYAGQ LRGRVKQQFA TMGERRRFWE 

       190        200        210        220        230        240 
KLFVNDRLAQ SLANNDQKAI TETTEQLINE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ 

       250        260        270        280        290        300 
QADVVVYDRL VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK 

       310        320        330        340        350        360 
GGDPFIFGRG GEELETLCNA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA QSVRLITGHL 

       370        380        390        400        410        420 
KTGGELDWEN LAAEKQTLVF YMGLNQAATI QQKLIEHGMP GEMPVAIVEN GTAVTQRVID 

       430        440        450 
GTLTQLGELA QQMNSPSLII IGRVVGLRDK LNWFSNH 

P0AEA8 in FASTA format

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