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UniProtKB/Swiss-Prot entry P0AFD0


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NUOB_SHIFL
Primary accession number P0AFD0
Secondary accession numbers P33598 P78090 P78186 P78187
Integrated into Swiss-Prot on December 20, 2005
Sequence was last modified on December 20, 2005 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 23)
Name and origin of the protein
Protein name NADH-quinone oxidoreductase subunit B
Synonyms EC 1.6.99.5
NADH dehydrogenase I subunit B
NDH-1 subunit B
Gene name
Name: nuoB
OrderedLocusNames: SF2363, S2498
From
Shigella flexneri [TaxID: 623] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Shigella.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=301 / Serotype 2a;
DOI=10.1093/nar/gkf566; PubMed=12384590 [NCBI, ExPASy, EBI, Israel, Japan]
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157.";
Nucleic Acids Res. 30:4432-4441(2002).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 700930 / 2457T / Serotype 2a;
DOI=10.1128/IAI.71.5.2775-2786.2003; PubMed=12704152 [NCBI, ExPASy, EBI, Israel, Japan]
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T.";
Infect. Immun. 71:2775-2786(2003).
Comments
  • FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).
  • CATALYTIC ACTIVITY: NADH + quinone = NAD+ + quinol.
  • COFACTOR: Binds 1 4Fe-4S cluster (Potential).
  • SUBUNIT: Composed of 13 different subunits (By similarity).
  • SIMILARITY: Belongs to the complex I 20 kDa subunit family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE005674; AAN43876.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE014073; AAP17694.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_708169.1; -.
NP_837884.1; -.
3D structure databases
ModBase P0AFD0.
Enzyme and pathway databases
BioCyc SFLE198214:AAN43876.1-MON; -.
Family and domain databases
InterPro IPR006138; NADH_DHase_20kDa_su.
IPR014406; NiFe_hyd_3_ssu/Q_oxred_NuoB.
IPR006137; OxRdtase_q6.
Graphical view of domain structure.
PANTHER PTHR11995:SF2; NADH_DH_20kDa; 1.
PTHR11995; NiFe_hyd_3_ssu/Q_oxred_NuoB; 1.
Pfam PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR01957; nuoB_fam; 1.
PROSITE PS01150; COMPLEX1_20K; 1.
BLOCKS P0AFD0.
Genome annotation databases
GeneID 1027233; -.
1078089; -.
GenomeReviews AE014073_GR; S2498.
AE005674_GR; SF2363.
KEGG sfl:SF2363; -.
sfx:S2498; -.
Phylogenomic databases
HOGENOM P0AFD0; -.
Genome annotation databases
CMR P0AFD0; SF2363.
Other
ProtoNet P0AFD0.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
4Fe-4S; Complete proteome; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase; Quinone; Ubiquinone.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   220  220     NADH-quinone oxidoreductase subunit B. PRO_0000118768
METAL   63    63        Iron-sulfur (4Fe-4S) (Potential). 
METAL   64    64        Iron-sulfur (4Fe-4S) (Potential). 
METAL   129   129        Iron-sulfur (4Fe-4S) (Potential). 
METAL   158   158        Iron-sulfur (4Fe-4S) (Potential). 
Sequence information
Length: 220 AA [This is the length of the unprocessed precursor] Molecular weight: 25056 Da [This is the MW of the unprocessed precursor] CRC64: EBD6268505993880 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDYTLTRIDP NGENDRYPLQ KQEIVTDPLE QEVNKNVFMG KLNDMVNWGR KNSIWPYNFG 

        70         80         90        100        110        120 
LSCCYVEMVT SFTAVHDVAR FGAEVLRASP RQADLMVVAG TCFTKMAPVI QRLYDQMLEP 

       130        140        150        160        170        180 
KWVISMGACA NSGGMYDIYS VVQGVDKFIP VDVYIPGCPP RPEAYMQALM LLQESIGKER 

       190        200        210        220 
RPLSWVVGDQ GVYRANMQSE RERKRGERIA VTNLRTPDEI 

P0AFD0 in FASTA format

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