[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15. PLASMID=IncFI ColV3-K30; PubMed=3275632 [NCBI, ExPASy, EBI, Israel, Japan] Herrero M., de Lorenzo V., Neilands J.B.; "Nucleotide sequence of the iucD gene of the pColV-K30 aerobactin operon and topology of its product studied with phoA and lacZ gene fusions."; J. Bacteriol. 170:56-64(1988).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PLASMID=IncFI ColV3-K30; Marrone L., Viswanatha T.; Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF PRO-14. PLASMID=ColV-K311; STRAIN=K-311; DOI=10.1515/BC.1999.006; PubMed=10064136 [NCBI, ExPASy, EBI, Israel, Japan] Stehr M., Smau L., Singh M., Seth O., Macheroux P., Ghisla S., Diekmann H.; "Studies with lysine N6-hydroxylase. Effect of a mutation in the assumed FAD binding site on coenzyme affinities and on lysine hydroxylating activity."; Biol. Chem. 380:47-54(1999).

Comments

FUNCTION: This is one of the proteins involved in the biosynthesis of siderophores (such as the dihydroxamate siderophore, aerobactin), which are chelators that mediate the high-affinity iron transport systems induced by the organism under iron-stressed conditions.
CATALYTIC ACTIVITY: L-lysine + NADPH + O₂ = N⁶-hydroxy-L-lysine + NADP⁺ + H₂O.
COFACTOR: FAD (By similarity).
PATHWAY: Siderophore biosynthesis; aerobactin biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm. Cell membrane.
SIMILARITY: Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M18968; AAA23196.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U90207; AAC00523.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF016586; AAB71391.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF016587; AAB71392.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A28665; BVECID.

3D structure databases

ModBase

P11295.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-11590; -.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0047091;	Molecular function: L-lysine 6-monooxygenase (NADPH) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Cytoplasm; Direct protein sequencing; FAD; Flavoprotein; Ion transport; Iron; Iron transport; Membrane; NADP; Oxidoreductase; Plasmid; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 425`	`425`	`L-lysine 6-monooxygenase.`	`PRO_0000204028`
`NP_BIND`	`8 14`	`7`	`FAD (Potential).`
`MUTAGEN`	`14 14`		`P->G: Low activity.`
`CONFLICT`	`139 139`		`E -> Q (in Ref. 1; AAA23196).`
`CONFLICT`	`172 172`		`M -> S (in Ref. 1; AAA23196).`
`CONFLICT`	`231 231`		`E -> D (in Ref. 1; AAA23196).`
`CONFLICT`	`258 267`		`MTSDGITADS -> TDIGWHHCPIL (in Ref. 1; AAA23196).`

Sequence information

Length: 425 AA [This is the length of the unprocessed precursor]

Molecular weight: 48726 Da [This is the MW of the unprocessed precursor]

CRC64: 0F8B2A2F87E39751 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKSVDFIGV GTGPFNLSIA ALSHQIEELD CLFFDEHPHF SWHPGMLVPD CHMQTVFLKD 

        70         80         90        100        110        120 
LVSAVAPTNP YSFVNYLVKH KKFYRFLTSR LRTVSREEFS DYLRWAAEDM NNLYFSHTVE 

       130        140        150        160        170        180 
NIDFDKKRRL FLVQTSQGEY FARNICLGTG KQPYLPPCVK HMTQSCFHAS EMNLRRPDLS 

       190        200        210        220        230        240 
GKRITVVGGG QSGADLFLNA LRGEWGEAAE INWVSRRNNF NALDEAAFAD EYFTPEYISG 

       250        260        270        280        290        300 
FSGLEEDIRH QLLDEQKMTS DGITADSLLT IYRELYHRFE VLRKPRNIRL LPSRSVTTLE 

       310        320        330        340        350        360 
SSGPGWKLLM EHHLDQGRES LESDVVIFAT GYRSALPQIL PSLMPLITMH DKNTFKVRDD 

       370        380        390        400        410        420 
FTLEWSGPKE NNIFVVNASM QTHGIAEPQL SLMAWRSARI LNRVMGRDLF DLSMPPALIQ 


WRSGT

P11295 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools