[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=3170489 [NCBI, ExPASy, EBI, Israel, Japan] Brunel F., Davison J.; "Cloning and sequencing of Pseudomonas genes encoding vanillate demethylase."; J. Bacteriol. 170:4924-4930(1988).

Comments

CATALYTIC ACTIVITY: Vanillate + O₂ + NADH = 3,4-dihydroxybenzoate + NAD⁺ + H₂O + formaldehyde.
COFACTOR: Binds 1 2Fe-2S cluster (Probable).
COFACTOR: Binds 1 iron ion (Probable).
PATHWAY: Xenobiotic degradation; vanillyl-alcohol degradation .
SUBUNIT: This demethylase system consists of two proteins: an oxygenase and an oxygenase reductase.
SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase alpha subunit family.
SIMILARITY: Contains 1 Rieske domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M22077; AAA26019.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P12609.

Family and domain databases

InterPro

IPR005806; Rieske_reg.
IPR015881; Ring-hydroxy_dOase_2Fe2S_BS.
Graphical view of domain structure.

Gene3D

G3DSA:2.102.10.10; Rieske_reg; 1.

Pfam

PF00355; Rieske; 1.
Pfam graphical view of domain structure.

PROSITE

PS51296; RIESKE; 1.
PS00570; RING_HYDROXYL_ALPHA; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

2Fe-2S; Aromatic hydrocarbons catabolism; Iron; Iron-sulfur; Lignin degradation; Metal-binding; Monooxygenase; NAD; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 329`	`329`	`Vanillate O-demethylase oxygenase subunit.`	`PRO_0000085058`
`DOMAIN`	`1 84`	`84`	`Rieske.`
`METAL`	`24 24`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`26 26`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`
`METAL`	`43 43`		`Iron-sulfur (2Fe-2S) (By similarity).`
`METAL`	`46 46`		`Iron-sulfur (2Fe-2S); via pros nitrogen (By similarity).`

Sequence information

Length: 329 AA [This is the length of the unprocessed precursor]

Molecular weight: 36579 Da [This is the MW of the unprocessed precursor]

CRC64: BAEA8D469E92BF43 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MICNERMVIY RGAGQRVAAL EDFCPHRGAP LSLGSIQDGK LVCGYHGLVM DCDGRTASMP 

        70         80         90        100        110        120 
AQRVQAFPCI RAFPAQERHG FIWVWPGDAA LADPALIPHL EWAENPAWAY GGGLYHIACD 

       130        140        150        160        170        180 
YRLMIDNLMD LTHETYVHAS SIGQKEIDEA PVSTRVEGDR LITGRFMEGI LAPPFWRAAL 

       190        200        210        220        230        240 
RGNGLADDVP VDRWQICRFT PPSHVLIEVG VAHAGRGGYD APADCKASSI VVDFITPETD 

       250        260        270        280        290        300 
TSIWYFWGMA RSFRPEDNEL TARIREGQGT IFAEDLEMLE QQQRNLLAWP ERPLLKLNID 

       310        320 
AGGVQSRRII ERLVSAERAA EAQLIGRQA

P12609 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools