NUCLEOTIDE SEQUENCE.
STRAIN=cv. Rosakrone;
TISSUE=Seedling;
PubMed=2247465 [NCBI, ExPASy, EBI, Israel, Japan]
Liaud M.-F., Zhang D.-X., Cerff R.;
"Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus.";
Proc. Natl. Acad. Sci. U.S.A. 87:8918-8922(1990).

[2]

NUCLEOTIDE SEQUENCE [MRNA] OF 5-451.
STRAIN=cv. Rosakrone;
TISSUE=Seedling;
DOI=10.1007/BF00027337; PubMed=2562762 [NCBI, ExPASy, EBI, Israel, Japan]
Brinkmann H., Cerff R., Salomon M., Soll J.;
"Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach.";
Plant Mol. Biol. 13:81-94(1989).

Comments

CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NADP⁺ = 3-phospho-D-glyceroyl phosphate + NADPH.
PATHWAY: Carbohydrate biosynthesis; Calvin cycle .
SUBUNIT: Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).
SUBCELLULAR LOCATION: Plastid, chloroplast.
MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M55147; AAA84543.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X15188; CAA33262.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S16507; DEPMNB.

3D structure databases

HSSP

P19866; 1NBO. [HSSP ENTRY / PDB]

SMR

P12859; 85-422.

ModBase

P12859.

Ontologies

GO:0047100;	Molecular function: glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR000173; GlycerAld_3-P_DHase.
IPR006424; Glyceraldehyde-3-P_DHase_1.
Graphical view of domain structure.

PANTHER

PTHR10836; GAP_DH; 1.

Pfam

PF02800; Gp_dh_C; 1.
PF00044; Gp_dh_N; 1.
Pfam graphical view of domain structure.

PRINTS

PR00078; G3PDHDRGNASE.

TIGRFAMs

TIGR01534; GAPDH-I; 1.

PS00071; GAPDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calvin cycle; Chloroplast; NADP; Oxidoreductase; Plastid; Transit peptide.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 84`	`84`	`Chloroplast.`
`CHAIN`	`85 451`	`367`	`Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic.`	`PRO_0000010424`
`NP_BIND`	`95 96`	`2`	`NADP (By similarity).`
`REGION`	`238 240`	`3`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`REGION`	`297 298`	`2`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`ACT_SITE`	`239 239`		`Nucleophile (By similarity).`
`BINDING`	`119 119`		`NADP (By similarity).`
`BINDING`	`164 164`		`NADP; via carbonyl oxygen (By similarity).`
`BINDING`	`269 269`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`284 284`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`320 320`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`403 403`		`NADP (By similarity).`
`SITE`	`266 266`	`1`	`Activates thiol group during catalysis (By similarity).`
`CONFLICT`	`255 255`		`F -> S (in Ref. 2; CAA33262).`

Sequence information

Length: 451 AA [This is the length of the unprocessed precursor]

Molecular weight: 48097 Da [This is the MW of the unprocessed precursor]

CRC64: C5D63CE37B19CE57 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATHAALAST RIPTNTRFPS KTSHSFPSQC ASKRLEVGEF SGLKSTSCIS YVHSARDSSF 

        70         80         90        100        110        120 
YDVVAAQLTS KANGSTAVKG VTVAKLKVAI NGFGRIGRNF LRCWHGRKDS PLEVIVVNDS 

       130        140        150        160        170        180 
GGVKNASHLL KYDSMLGTFK AEVKILNNET ITVDGKPIKV VSSRDPLKLP WAELGIDIVI 

       190        200        210        220        230        240 
EGTGVFVDGP GAGKHIQAGA KKVIITAPAK GADIPTYVIG VNEQDYGHEV ADIISNASCT 

       250        260        270        280        290        300 
TNCLAPFAKV LDEEFGIVKG TMTTTHSYTG DQRLLDASHR DLRRARAAAL NIVPTSTGAA 

       310        320        330        340        350        360 
KAVSLVLPQL KGKLNGIALR VPTPNVSVVD LVVNVAKKGI SAEDVNAAFR KAAEGPLKGI 

       370        380        390        400        410        420 
LDVCDVPLVS VDFRCSDVST TIDSSLTMVM GDDMVKVVAW YDNEWGYSQR VVDLAHLVAN 

       430        440        450 
KWPGTPKVGS GDPLEDFCET NPADEECKVY E

P12859 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools