[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=3316183 [NCBI, ExPASy, EBI, Israel, Japan] Menon N.K., Peck H.D. Jr., le Gall J., Przybyla A.E.; "Cloning and sequencing of the genes encoding the large and small subunits of the periplasmic (NiFeSe) hydrogenase of Desulfovibrio baculatus."; J. Bacteriol. 169:5401-5407(1987).
[2]	ERRATUM, AND SEQUENCE REVISION. Menon N.K., Pect H.D. Jr., le Gall J., Przybyla A.E.; J. Bacteriol. 170:4429-4429(1988).
[3]	PROTEIN SEQUENCE OF 33-67. STRAIN=DSM 1743; DOI=10.1016/0006-291X(87)90376-7; PubMed=3322275 [NCBI, ExPASy, EBI, Israel, Japan] Prickril B.C., He S.H., Li C., Menon N.K., Choi E.S., Przybyla A.E., Dervartanian D.V., Peck H.D. Jr., Fauque G., le Gall J., Teixeira M., Moura I., Moura J.J.G., Patil D., Huynh B.H.; "Identification of three classes of hydrogenase in the genus, Desulfovibrio."; Biochem. Biophys. Res. Commun. 149:369-377(1987).
[4]	X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS). DOI=10.1016/S0969-2126(99)80072-0; PubMed=10378275 [NCBI, ExPASy, EBI, Israel, Japan] Garcin E., Vernede X., Hatchikian E.C., Volbeda A., Frey M., Fontecilla-Camps J.-C.; "The crystal structure of a reduced [NiFeSe] hydrogenase provides an image of the activated catalytic center."; Structure 7:557-566(1999).

Comments

CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 3 4Fe-4S clusters. Cluster 1 is referred to as proximal, cluster 2 as distal, cluster 3 as medial.
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Periplasm.
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M18271; AAA23376.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A28380; HQDVSB.

3D structure databases

PDB

1CC1; X-ray; 2.15 A; S=33-315.

[ExPASy / RCSB / EBI]

PDBsum

1CC1; -.

ModBase

P13063.

Protein-protein interaction databases

DIP

DIP:6126N; -.

Family and domain databases

InterPro

IPR001821; NiFe_hyd_ssu.
IPR013634; NiFe_hyd_ssu_N.
IPR006137; OxRdtase_q6.
IPR006311; Tat.
Graphical view of domain structure.

Pfam

PF08425; NiFe_dehyd_N; 1.
PF01058; Oxidored_q6; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000310; NiFe_hyd_ssu; 1.

PRINTS

PR00614; NIHGNASESMLL.

TIGRFAMs

TIGR00391; hydA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; 4Fe-4S; Direct protein sequencing; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Periplasm; Signal.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 32`	`32`	`Tat-type signal.`
`CHAIN`	`33 315`	`283`	`Periplasmic [NiFeSe] hydrogenase small subunit.`	`PRO_0000013420`
`METAL`	`50 50`		`Iron-sulfur 1 (4Fe-4S).`
`METAL`	`53 53`		`Iron-sulfur 1 (4Fe-4S).`
`METAL`	`158 158`		`Iron-sulfur 1 (4Fe-4S).`
`METAL`	`196 196`		`Iron-sulfur 1 (4Fe-4S).`
`METAL`	`240 240`		`Iron-sulfur 2 (4Fe-4S); via pros nitrogen.`
`METAL`	`243 243`		`Iron-sulfur 2 (4Fe-4S).`
`METAL`	`263 263`		`Iron-sulfur 2 (4Fe-4S).`
`METAL`	`269 269`		`Iron-sulfur 2 (4Fe-4S).`
`METAL`	`278 278`		`Iron-sulfur 3 (4Fe-4S).`
`METAL`	`290 290`		`Iron-sulfur 3 (4Fe-4S).`
`METAL`	`296 296`		`Iron-sulfur 3 (4Fe-4S).`
`METAL`	`299 299`		`Iron-sulfur 3 (4Fe-4S).`
`STRAND`	`40 48`	`9`
`HELIX`	`52 58`	`7`
`TURN`	`61 64`	`4`
`HELIX`	`65 71`	`7`
`STRAND`	`73 77`	`5`
`TURN`	`79 81`	`3`
`HELIX`	`86 99`	`14`
`TURN`	`100 102`	`3`
`STRAND`	`103 113`	`11`
`HELIX`	`115 118`	`4`
`STRAND`	`121 124`	`4`
`STRAND`	`131 133`	`3`
`HELIX`	`138 145`	`8`
`HELIX`	`146 148`	`3`
`STRAND`	`149 156`	`8`
`HELIX`	`157 161`	`5`
`HELIX`	`164 166`	`3`
`HELIX`	`177 184`	`8`
`STRAND`	`190 193`	`4`
`HELIX`	`200 215`	`16`
`TURN`	`217 219`	`3`
`HELIX`	`232 235`	`4`
`STRAND`	`236 238`	`3`
`TURN`	`239 242`	`4`
`HELIX`	`246 250`	`5`
`STRAND`	`260 264`	`5`
`HELIX`	`265 267`	`3`
`HELIX`	`271 273`	`3`
`HELIX`	`278 281`	`4`
`TURN`	`284 287`	`4`
`HELIX`	`290 293`	`4`
`TURN`	`302 305`	`4`
`HELIX`	`306 308`	`3`

Sequence information

Length: 315 AA [This is the length of the unprocessed precursor]

Molecular weight: 34221 Da [This is the MW of the unprocessed precursor]

CRC64: A3C592F12B95ED83 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSLSRREFVK LCSAGVAGLG ISQIYHPGIV HAMTEGAKKA PVIWVQGQGC TGCSVSLLNA 

        70         80         90        100        110        120 
VHPRIKEILL DVISLEFHPT VMASEGEMAL AHMYEIAEKF NGNFFLLVEG AIPTAKEGRY 

       130        140        150        160        170        180 
CIVGETLDAK GHHHEVTMME LIRDLAPKSL ATVAVGTCSA YGGIPAAEGN VTGSKSVRDF 

       190        200        210        220        230        240 
FADEKIEKLL VNVPGCPPHP DWMVGTLVAA WSHVLNPTEH PLPELDDDGR PLLFFGDNIH 

       250        260        270        280        290        300 
ENCPYLDKYD NSEFAETFTK PGCKAELGCK GPSTYADCAK RRWNNGINWC VENAVCIGCV 

       310 
EPDFPDGKSP FYVAE

P13063 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools