NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2670935 [NCBI, ExPASy, EBI, Israel, Japan]
Peoples O.P., Sinskey A.J.;
"Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase.";
J. Biol. Chem. 264:15293-15297(1989).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nbt1244; PubMed=16964242 [NCBI, ExPASy, EBI, Israel, Japan]
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16.";
Nat. Biotechnol. 24:1257-1262(2006).

Comments

CATALYTIC ACTIVITY: (R)-3-hydroxyacyl-CoA + NADP⁺ = 3-oxoacyl-CoA + NADPH.
PATHWAY: Biopolymer metabolism; poly-(R)-3-hydroxybutyric acid biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

J04987; AAA21973.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AM260479; CAJ92574.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B34340; RDALAE.

RefSeq

YP_725942.1; -.

3D structure databases

HSSP

P97852; 1GZ6. [HSSP ENTRY / PDB]

ModBase

P14697.

Family and domain databases

InterPro

IPR011283; Acetoacetyl-CoA_reductase.
IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

TIGRFAMs

TIGR01829; AcAcCoA_reduct; 1.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

P14697.

Genome annotation databases

GeneID

4249784; -.

GenomeReviews

AM260479_GR; H16_A1439.

KEGG

reh:H16_A1439; -.

Phylogenomic databases

HOGENOM

P14697; -.

Genome annotation databases

CMR

P14697; H16_A1439.

Other

ProtoNet

P14697.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; PHB biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 246`	`246`	`Acetoacetyl-CoA reductase.`	`PRO_0000054748`
`NP_BIND`	`8 32`	`25`	`NADP (By similarity).`
`ACT_SITE`	`153 153`		`Proton acceptor (By similarity).`
`BINDING`	`140 140`		`Substrate (By similarity).`

Sequence information

Length: 246 AA [This is the length of the unprocessed precursor]

Molecular weight: 26370 Da [This is the MW of the unprocessed precursor]

CRC64: AD6739E0F5C93C06 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTQRIAYVTG GMGGIGTAIC QRLAKDGFRV VAGCGPNSPR REKWLEQQKA LGFDFIASEG 

        70         80         90        100        110        120 
NVADWDSTKT AFDKVKSEVG EVDVLINNAG ITRDVVFRKM TRADWDAVID TNLTSLFNVT 

       130        140        150        160        170        180 
KQVIDGMADR GWGRIVNISS VNGQKGQFGQ TNYSTAKAGL HGFTMALAQE VATKGVTVNT 

       190        200        210        220        230        240 
VSPGYIATDM VKAIRQDVLD KIVATIPVKR LGLPEEIASI CAWLSSEESG FSTGADFSLN 


GGLHMG

P14697 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools