[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=ATCC 33303 / B10; DOI=10.1007/BF00340186; PubMed=3067084 [NCBI, ExPASy, EBI, Israel, Japan] Leclerc M., Colbeau A., Cauvin B., Vignais P.M.; "Cloning and sequencing of the genes encoding the large and the small subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus."; Mol. Gen. Genet. 214:97-107(1988).
[2]	ERRATUM, AND SEQUENCE REVISION. Leclerc M., Colbeau A., Cauvin B., Vignais P.M.; Mol. Gen. Genet. 215:368-368(1989).

Comments

FUNCTION: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions.
CATALYTIC ACTIVITY: H₂ + A = AH₂.
COFACTOR: Binds 1 nickel ion per subunit (By similarity).
SUBUNIT: Heterodimer of a large and a small subunit.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X13520; CAA31870.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S08317; S08317.

3D structure databases

HSSP

P12944; 2FRV. [HSSP ENTRY / PDB]

ModBase

P15284.

Family and domain databases

InterPro

IPR001501; Ni-dep_hyd_lsu.
Graphical view of domain structure.

Pfam

PF00374; NiFeSe_Hases; 1.
Pfam graphical view of domain structure.

PROSITE

PS00507; NI_HGENASE_L_1; 1.
PS00508; NI_HGENASE_L_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Membrane; Metal-binding; Nickel; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 597`	`597`	`Uptake hydrogenase large subunit.`	`PRO_0000199716`
`METAL`	`75 75`		`Nickel (Potential).`
`METAL`	`78 78`		`Nickel (Potential).`
`METAL`	`576 576`		`Nickel (Potential).`
`METAL`	`579 579`		`Nickel (Potential).`

Sequence information

Length: 597 AA [This is the length of the unprocessed precursor]

Molecular weight: 66046 Da [This is the MW of the unprocessed precursor]

CRC64: C038E25E05EFB9C8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTTQTPNGFT LDNAGKRIVV DPVTRIEGHM RCEVNVNDQG IITNAVSTGT MWRGLEVILK 

        70         80         90        100        110        120 
GRDPRDAWAF TERICGVCTG THALTSVRAV ESALGITIPD NANSIRNMMQ LNLQIHDHIV 

       130        140        150        160        170        180 
HFYHLHALDW VNPVNALRAD PKATSELQQM VSPSHPLSSP GYFRDVQNRL KKFVESGQLG 

       190        200        210        220        230        240 
LFKNGYWDNP AYKLPPEADL MATTHYLEAL DLQKEVVKVH TIFGGKNPHP NWLVGGVPCP 

       250        260        270        280        290        300 
INVDGVGAVG AINMERLNLV SSIIDRCTEF TRNVYLPDLK AIGGFYKEWL YGGGLSGQSV 

       310        320        330        340        350        360 
LSYGDIPENP NDFSAGQLHL PRGAIINGNL NEVHDVDTTD PEQVQEFVDH SWYDYGEPGM 

       370        380        390        400        410        420 
GLHPWDGRTE PKFELGPNLK GTRTNIENID EGAKYSWIKA PRWRGNAMEV GPLAATSSVT 

       430        440        450        460        470        480 
RKGHEDIKNQ VEGLLRDMNL PVSALFSTLG RTAARALEAE YCCRLQKHFF DKLVTNIKNG 

       490        500        510        520        530        540 
DSSTANVEKW DPSTWPKEAK GVGMTEAPRG ALGHWVKIKD GRIENYQCVV PTTWNGSPRD 

       550        560        570        580        590 
SKGNIGAFEA SLLNTKMERP EEPVEILRTL HSFDPCLACS THVMSAEGAP LTTVKVR

P15284 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools